Currently,most urban sewage suffers from insufficient internal carbon sources,and additional carbon source needs to be added to meet the metabolic needs of microorgan-isms.Compared to traditional carbon sources,the new biomass carbon source has high envi-ronmental safety,remarkable denitrification effect.Biomass corn straw was used as raw mate-rial to prepare biomass straw fermentation liquid by anaerobic fermentation technology.The effects of different solid-liquid ratio and fermentation temperature on the chemical oxygen demand(COD)content of carbon source were investigated,and the optimal fermentation pro-cess conditions were obtained.High performance liquid chromatography(HPLC),spectropho-tometry and 16S high-throughput sequencing were used to explain the acid production mechanism of biomass fermentation solution.The denitrification experiment was carried out under the optimal C/N,and all the effluent indexes met the grade A standard of urban sewage discharge,indicating that the prepared biomass carbon source had good denitrifica-tion performance.