石油化工应用2024,Vol.43Issue(10) :82-88,95.DOI:10.3969/j.issn.1673-5285.2024.10.018

废弃秸秆制备发酵液碳源实验研究

Experimental study on carbon source of fermentation solution prepared from waste straw

张永 刘明雨 孙李平 陈爽 朱晓倩 张一凡
石油化工应用2024,Vol.43Issue(10) :82-88,95.DOI:10.3969/j.issn.1673-5285.2024.10.018

废弃秸秆制备发酵液碳源实验研究

Experimental study on carbon source of fermentation solution prepared from waste straw

张永 1刘明雨 2孙李平 1陈爽 2朱晓倩 2张一凡2
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作者信息

  • 1. 东营市鲁辰水务有限责任公司,山东 东营 257091;中国石化集团胜利石油管理局有限公司供水分公司,山东 东营 257000
  • 2. 中国石油大学(华东)化学化工学院,山东 青岛 266580
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摘要

当前城镇污水在生物脱氮过程中大多存在内部碳源不足问题,需额外添加碳源,以满足微生物的代谢需求.新型生物质碳源相比较传统碳源环境安全性高、脱氮效果显著.该实验以生物质废弃玉米秸秆为原料,采用厌氧发酵方法制备生物质发酵液,设计了不同固液比和发酵温度对碳源化学需氧量(COD)等评价指标的影响,得出最优发酵工艺条件.利用高效液相色谱(HPLC)、分光光度法、16S高通量测序等对生物质发酵液产酸机理进行解释.在最佳C/N下进行反硝化实验,出水各项指标均满足城镇污水排放一级A标准,说明制备的生物质碳源具有良好的反硝化性能.

Abstract

Currently,most urban sewage suffers from insufficient internal carbon sources,and additional carbon source needs to be added to meet the metabolic needs of microorgan-isms.Compared to traditional carbon sources,the new biomass carbon source has high envi-ronmental safety,remarkable denitrification effect.Biomass corn straw was used as raw mate-rial to prepare biomass straw fermentation liquid by anaerobic fermentation technology.The effects of different solid-liquid ratio and fermentation temperature on the chemical oxygen demand(COD)content of carbon source were investigated,and the optimal fermentation pro-cess conditions were obtained.High performance liquid chromatography(HPLC),spectropho-tometry and 16S high-throughput sequencing were used to explain the acid production mechanism of biomass fermentation solution.The denitrification experiment was carried out under the optimal C/N,and all the effluent indexes met the grade A standard of urban sewage discharge,indicating that the prepared biomass carbon source had good denitrifica-tion performance.

关键词

发酵/生活污水/碳源/反硝化

Key words

fermentation/domestic sewage/carbon source/denitrification

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基金项目

中国石油大学(华东)校教改重点项目(CZ2022008)

出版年

2024
石油化工应用
宁夏化工学会

石油化工应用

影响因子:0.276
ISSN:1673-5285
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