Study on comparison of methods of extracting genome DNA from wheat
This paper introduces 4 methods of wheat DNA extraction. The DNA concentration and quality are compared by using agarose gel electrophoresis and protein nucteic acid analyzer. Experimental results show that there is somewhat difference in DNA concentration between the 4 methods. The DNA concentration in Program I is the highest, but the consumption of reagent is the most. The DNA concentration of Program 11 is the lowest, but the process is simple. The extracted DNA concentrations are equivalent to Programs Ⅲ and IV. There is protein pollution in Program Ⅲ, but the process is simple. The purity of Program IV is higher than others, but the process is the most complex, Using the same pair of primers and the reaction system, the extracted DNA is amplified by PCR. The results show that the 4 methods all can meet the requirement of molecular marker detection.
NETL
NSTL
万方数据
维普
