首页|桦褐孔菌β-葡萄糖苷酶基因的克隆与定量表达分析

桦褐孔菌β-葡萄糖苷酶基因的克隆与定量表达分析

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利用反转录PCR(reverse transcription PCR,RT-PCR)和cDNA末端快速扩增(rapid-amplificationof cDNA ends,RACE)技术从桦褐孔菌(Inonotus obliquus)菌核中克隆了β-葡萄糖苷酶基因的全长DNA序列,命名为IO-BGL.该DNA序列全长3382 bp,其中开放阅读框(ORF)长度为2583 bp,含有13个内含子和14个外显子,编码860个氨基酸,相对分子量为93.4 kD,等电点为5.57,编码的氨基酸序列与地中海嗜蓝孢孔菌(Fomitiporia mediterranea)相似性最高(85％).采用实时荧光定量PCR技术研究IO-BGL基因在菌核发育过程中的表达量变化,结果表明,IO-BGL基因在菌核的发育过程中,表达量呈先上升后下降趋势.
Cloning and Quantitative Expression of a β-Glucosidase Gene from Inonotus obliquus
The full-length DNA sequence of a β-glucosidase gene (IO-BGL) was cloned from Inonotus obliquus sclerotia by RT-PCR and RACE.The gene had a molecular mass of 93.4 kD, a pI value of 5.57, and consisted of 3382 bp, 13 introns and 14 exons, and an open reading frame (ORF) of 2583 bp that encoded a protein of 860 amino acids.The putative amino acid sequence of IO-BGL was 85％ similar to a β-glucosidase from Fomitiporia mediterranea.Real time quantitative PCR revealed that IO-BGL expression increased during the first 170 days of sclerotium development and then decreased.

Inonotus obliquusβ-glucosidasesclerotium

隋飞飞、陈艳秋

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延边大学农学院,吉林延吉133002

桦褐孔菌 内切葡聚糖酶 菌核

国家自然科学基金

31160408

2015

10.16488/j.cnki.1005-9873.2015.03.001

食用菌学报
上海市农业科学院食用菌研究所,上海市农业科学院农业科技信息研究所

食用菌学报

CSTPCDCSCD北大核心
影响因子：0.771
ISSN：1005-9873
年,卷(期)：2015.22(3)
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