Cloning and Expression Analysis of Trehalose-6-Phosphate Synthase from Sanghuangporus vaninii
The trehalose 6-phosphate synthase(SvTPS)gene of Sanghuangporus vaninii was cloned,analyzed by bioinformatics and heterologously expressed in E.coli.The expression of SvTPS in mycelia and different ages of fruiting bodies was measured by fluorescence quantitative PCR,and the activity of SvTPS was also determined.The results showed that SvTPS has an open reading frame of 1 894 bp,encoding 576 amino acids.The molecular weight,theoretical isoelectric point(pl),aliphatic index,grand average of hydrophilicity(GRAVY),instability index of SvTPS were estimated to be 65 130,6.21,88.14,-0.281 and 42.41,respectively.The amino acid sequence of SvTPS has the highest similarity with S.baumii,having only one TPS domain.In terms of the secondary structure of SvTPS,a-helix,β-sheet,extended strand and random coil account for 48.61％,5.56％,15.62％and 30.21％,respectively.The expression level of SvTPS was the highest in three-year-old fruiting bodies and the lowest in mycelia.SvTPS activity was the lowest in mycelia,and increased with the increase of cultivation time,reaching the highest in three-year-old fruiting bodies.The results provided a reference for further exploring the role of SvTPS in the growth and development of S.vaninii.
万方数据
维普
