摘要
为筛选南方黑木耳(Auricularia heimuer)栽培时接种后的菌袋在遮阳棚内的最佳摆放模式,在初始摆放模式为"一"字型条件下,测量"一"字型不翻堆(Ⅰ)、"一"字型翻堆(Ⅱ)、"#"字型不翻堆(Ⅲ)不同部位(顶层、中层和底层)菌袋内的温度和产量;测定20、25、30、35、40 ℃黑木耳菌丝在培养皿内的生长速度和与菌丝活力相关的代谢酶(漆酶和脱氢酶)活性.结果显示:在实验范围内,Ⅰ、Ⅱ、Ⅲ的中层和底层菌袋日低温平均为30、28～31、24 ℃,菌袋间温度稳定性从高到低依次为Ⅰ、Ⅱ、Ⅲ;平均单袋产量从高到低依次为Ⅰ、Ⅱ、Ⅲ;在30、35℃时,菌丝生长速度较快,酶活性较高;"一"字型不翻堆模式有助于维持发菌期间菌袋的温度稳定性,其菌丝活力和产量较高.
Abstract
The optimal placement mode for Auricularia heimuer mushroom cultivation bags to grow in a shaded greenhouse was explored.Three placement modes,"一"shape placement without moving(Ⅰ),"一"shape placement with moving the top bags to the bottom 15 d after inoculation(Ⅱ),and"#"shape placement without moving(Ⅲ),were measured for bag temperature at different positions(top,middle,and bottom)and A.heimuer yield.The growth rate and activities of laccase and dehydrogenase of A.heimuer hyphae cultivated at 20 ℃,25 ℃,30 ℃,35 ℃,40 ℃ in Petri dish were also measured.The results show that the average daily minimum temperatures of the middle and bottom bags of Ⅰ,Ⅱ and Ⅲ were 30 ℃,28-31 ℃ and 24 ℃,respectively.The bag temperature stability from high to low was Ⅰ,Ⅱ,Ⅲ.The average yield per bag from high to low was Ⅰ,Ⅱ,Ⅲ.Mycelial growth rate and enzyme activities were higher at 30 ℃ and 35 ℃ than at other temperatures.The placement mode I was beneficial for maintaining temperature stability of cultivation bags,resulting in high mycelial vitality and high yield of A.heimuer.
基金项目
福建省星火项目(2021S0030)
福建省尤溪县食用菌产业集群建设项目(尤农[2022]94号)
福建省尤溪县食用菌产业集群建设项目(尤农[2023]92号)
福建省级科技特派员项目()
维普
万方数据