Effects of Substrate Nitrogen Content on Mycelial Growth,Enzyme Activity and Gene Expression Associated with Carbon and Nitrogen Metabolism in Stropharia rugosoannulata
To study the optimal nitrogen source for mycelial growth of Stropharia rugosoannulata,it was cultured under different nitrogen sources(corncob,wheat bran,rice bran,rapeseed cake,bean pulp and calces)and nitrogen contents(0.4％,0.6％,0.8％,1.0％,1.2％and 1.4％),and then measured for mycelial growth,enzyme activities and gene expression pertaining to nitrogen and carbon metabolism.The results showed that bean pulp powder was the optimal nitrogen source for mycelial growth.The mycelial growth rate of S.rugosoannulata was the greatest when the nitrogen content was 0.6％.Within the experimental range,the activity of nitrite reductase(NiR)in the control(0 nitrogen content)and the asparagine synthase(AS)activity of 1.2％nitrogen content were high.Both the activity and gene expression of glutamine-2-oxoglutarate aminotransferase(GOGAT)were the highest under 0.6％nitrogen content.For cellulose enzymes,the activity of endogenous β-1,4-glucanase(Cx)was the highest under 0.6％nitrogen content,and the activity of exogenous β-1,4-glucanase(Cl)was also high under 0.6％nitrogen content.The glutamate dehydrogenase gene was highly expressed under 1.2％nitrogen content.On the other hand,the expression levels of β-1,3-glucanase gene and hemicellulase(ACX)gene in the control were the highest.This study provided a reference for the development and application of liquid spawn of S.rugosoannulata.
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