Identification and Expression Analysis of PoLIM Family in Pleurotus ostreatus
The LIMs of Pleurotus ostreatus(PoLIMs)was searched in NCBI and JGI databases and subjected to bioinformatics analyses.Then the expression levels of the PoLIMs were determined at mycelium stage(7 d at 25 ℃),early twisting stage(5 d at 16 ℃),late twisting stage(10 d at 16 ℃),primordium stage(15 d at 16 ℃),and fruiting body stage(22 d at 16 ℃),respectively.The expression of PoLIMs in mycelium under cold stress of 4 ℃ was also determined at 12 h,24 h and 48 h,respectively.The results showed that PoLIM1-PoLIM6 contained 353-1 673 amino acids,and their isoelectric points ranged from 7.26 to 9.98.PoLIM1-PoLIM6 were predicted to be localized in the nucleus.There were 10 conserved motifs in PoLIM1-PoLIM6.Their promoter sequences contained a serial of response elements including light,auxin,MYB transcription factor,and low temperature stress.The expression of PoLIM1 was relatively high during the fruiting body stage,and that of PoLIM2 was relatively high during the late twisting stage and fruiting body stage.On the other hand,the expression of PoLIM3,PoLIM4,and PoLIM6 was relatively high during the mycelium stage.The expression of PoLIM5 was high during the late twisting stage,and then decreased during the primordium and fruiting body stages.Compared with the control(25 ℃),the cold stress at 4 ℃ resulted in an increase in the relative expression of PoLIM1,PoLIM3,PoLIM5,and PoLIM6.
NETL
NSTL
万方数据
