摘要
在NCBI、JGI数据库中检索糙皮侧耳(Pleurotus ostreatus)PoLIMs,进行生物信息学分析;测定PoLIM1～PoLIM6在25 ℃培养7 d(菌丝期),16℃培养5 d(扭结前期)、10 d(扭结后期)、15 d(原基期)、22 d(子实体期)的相对表达量以及菌丝在4℃冷胁迫12、24、48 h的相对表达量.结果表明:PoLIM1～PoLIM6的氨基酸数量为353～1 673;等电点为7.26～9.98;亚细胞定位预测均在细胞核中;有10个保守基序;启动子序列中包含光、生长素、MYB转录因子以及低温胁迫等响应元件.就相对表达量而言,PoLIMI在子实体期较高;PoLIM2在扭结后期和子实体期较高;PoLIM3、PoLIM4、PoLIM6在菌丝期较高;PoLIM5在扭结后期较高,在原基期及子实体期降低.与对照(25 ℃)相比,4 ℃冷胁迫时PoLIM1、PoLIM3、PoLIM5、PoLIM6的相对表达量升高.
Abstract
The LIMs of Pleurotus ostreatus(PoLIMs)was searched in NCBI and JGI databases and subjected to bioinformatics analyses.Then the expression levels of the PoLIMs were determined at mycelium stage(7 d at 25 ℃),early twisting stage(5 d at 16 ℃),late twisting stage(10 d at 16 ℃),primordium stage(15 d at 16 ℃),and fruiting body stage(22 d at 16 ℃),respectively.The expression of PoLIMs in mycelium under cold stress of 4 ℃ was also determined at 12 h,24 h and 48 h,respectively.The results showed that PoLIM1-PoLIM6 contained 353-1 673 amino acids,and their isoelectric points ranged from 7.26 to 9.98.PoLIM1-PoLIM6 were predicted to be localized in the nucleus.There were 10 conserved motifs in PoLIM1-PoLIM6.Their promoter sequences contained a serial of response elements including light,auxin,MYB transcription factor,and low temperature stress.The expression of PoLIM1 was relatively high during the fruiting body stage,and that of PoLIM2 was relatively high during the late twisting stage and fruiting body stage.On the other hand,the expression of PoLIM3,PoLIM4,and PoLIM6 was relatively high during the mycelium stage.The expression of PoLIM5 was high during the late twisting stage,and then decreased during the primordium and fruiting body stages.Compared with the control(25 ℃),the cold stress at 4 ℃ resulted in an increase in the relative expression of PoLIM1,PoLIM3,PoLIM5,and PoLIM6.
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