Transcriptome Mining of Genes Involved in Drought Stress Response in Morchella sextelata
Genes associated with drought stress in Morchella sextelata were identified by comparative transcriptome analysis.Two groups,control group and drought-stressed group,were established,and then three fruiting body samples(one per plot)were randomly taken from each group when fruiting bodies of the control group turned dark brown.Differentially expressed genes(DEGs)between the control and drought-stressed groups were analyzed through transcriptome sequencing and gene functional annotation.GO and KEGG enrichment analyses were used to identify significantly enriched GO terms and KEGG metabolic pathways.Based on the above analyses,key DEGs related to drought stress were determined,and then five key DEGs were randomly selected to be validated by qRT-PCR.The results showed that fruiting bodies in the control group were white to light yellow with a plump appearance,while those in the drought-stressed group were predominantly dark brown,shriveled,and smaller than the control group.A total of 2 817 DEGs were identified,including 1 492 up-regulated genes and 1 325 down-regulated genes.GO enrichment analysis showed that the DEGs were significantly enriched in DNA replication,DNA-dependant DNA replication,monosaccharide transport,protein-DNA complex,ribosome,MCM complex,structural constituent of ribosome,FAD binding,and solute:cation symporter activity,etc..KEGG enrichment analysis revealed the involvement of the DEGs in DNA replication,mismatch repair,steroid biosynthesis,ribosome base excision repair,nucleotide excision repair,methane metabolism,starch and sucrose metabolism,arginine and proline metabolism,and sulfur metabolism,etc..Eighteen key DEGs associated with drought stress were identified,including genes related to transporter proteins,heat shock proteins,transcription factors,kinases,and autophagy.The results of qRT-PCR were consistent with those of the transcriptome sequencing analysis for the five randomly selected DEGs.
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