Breeding of High Triterpenoid Content Ganoderma lucidum Strain'Hunong Lingzhi No.5'
Triterpenoids are a group of major biologically active substances in Ganoderma lucidum that have multiple biological activities,such as anti-tumor,anti-inflammation,hepatoprotection,neuroprotection,immunoregulation,hypolipidemic,hypoglycemic,anti-bacteria,and anti-aging effects.Breeding of G.lucidum cultivars with a high triterpenoid content is significantly important for the G.lucidum industry.Based on the Chinese cultivated Ganoderma germplasm resource information database,two strains,'Hunong Lingzhi No.1'(H1)cultivar with strong resistance to microorganisms and a high biological efficiency,and'Lingzhi G8'(G8)with a high triterpenoid content but weak resistance to microorganisms were selected as the parents to breed a new cultivar.Monokaryotic strains of H1 and G8 were prepared from their protoplasts respectively,and then crossed to yield 130 hybrids.Then,25 hybrids showing good myceial growth were selected for cultivation experiments and compared for proportion of harvestable cultivation bags,biological efficiency,fruiting body morphology and triterpenoid content.Eventually,strain Z18 was selected for showing superior traits compared with its parent strains.Compared with G8,Z18 showed a 66.67％increase in proportion of harvestable cultivation bags and a 22.54％increase in biological efficiency.Compared with H1,the triterpenoid content of Z18 increased by 51.26％.ERIC-PCR analysis showed that there were genetic differences between Z18 and its parents.Z18 passed the identification of non-major crop varieties in Shanghai and was named'Hunong Lingzhi No.5'.
Ganoderma lucidummonokaryotic strainshybridizationtriterpenoid contentyield per unit
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