摘要
为选育高产三萜灵芝(Ganoderma lucidum)新品种,以抗杂菌能力强、生物学效率高的'沪农灵芝1号'(H1)和三萜含量高、抗杂菌能力弱的'灵芝G8'(G8)为亲本,将原生质体单核菌株进行杂交,从130个杂交组合中筛选出菌丝长势较好的25个杂交菌株,并对其进行栽培试验,考察菌包成品率、生物学效率等农艺性状及子实体三萜含量,从中筛选出杂交菌株Z18.与亲本G8相比,Z18菌包成品率提高66.67％、生物学效率提高22.54％;与亲本H1相比,Z18子实体中三萜含量提高51.26％.ERIC-PCR分析结果显示,Z18与亲本存在遗传差异.Z18达到新品种审定标准,已通过上海市非主要农作物品种认定,定名为'沪农灵芝5号'.
Abstract
Triterpenoids are a group of major biologically active substances in Ganoderma lucidum that have multiple biological activities,such as anti-tumor,anti-inflammation,hepatoprotection,neuroprotection,immunoregulation,hypolipidemic,hypoglycemic,anti-bacteria,and anti-aging effects.Breeding of G.lucidum cultivars with a high triterpenoid content is significantly important for the G.lucidum industry.Based on the Chinese cultivated Ganoderma germplasm resource information database,two strains,'Hunong Lingzhi No.1'(H1)cultivar with strong resistance to microorganisms and a high biological efficiency,and'Lingzhi G8'(G8)with a high triterpenoid content but weak resistance to microorganisms were selected as the parents to breed a new cultivar.Monokaryotic strains of H1 and G8 were prepared from their protoplasts respectively,and then crossed to yield 130 hybrids.Then,25 hybrids showing good myceial growth were selected for cultivation experiments and compared for proportion of harvestable cultivation bags,biological efficiency,fruiting body morphology and triterpenoid content.Eventually,strain Z18 was selected for showing superior traits compared with its parent strains.Compared with G8,Z18 showed a 66.67％increase in proportion of harvestable cultivation bags and a 22.54％increase in biological efficiency.Compared with H1,the triterpenoid content of Z18 increased by 51.26％.ERIC-PCR analysis showed that there were genetic differences between Z18 and its parents.Z18 passed the identification of non-major crop varieties in Shanghai and was named'Hunong Lingzhi No.5'.
基金项目
上海市科委国内科技合作课题(22015820800)
西藏自治区重点研发计划(XZ202301ZY0007N)
国家科技期刊平台
NSTL
万方数据