Application of Single-Ascospore Strains Isolated from Morchella importuna Hybrids in Breeding of New Cultivars
Morchella importuna is one of the major cultivated morel species in China.However,the commercial cultivation of M.importuna has been troubled by strain degradation,declines in output,and failing to form fruiting bodies.To address these issues,progeny ascocarps from"wild × wild"hybrid combinations ZQW31×ZQW26(A)and ZQW22×ZQW10(B),and"artificial × wild"hybrid combinations 282×ZQW10(C)and 282×ZQW26(D)were used to yield a single-ascospore population.Based on mating type genes,a total of 145 single-ascospore strains were selected for fruiting experiments,and then evaluated for agronomic traits,such as ascospore germination ratio,fruiting yield,fruiting uniformity,fruiting period,ascocarp morphology and disease resistance.The results showed that the germination ratio of B was relatively high.Progenies of C and D had a relatively high yield.Most single ascospore strains showed higher yields at the 1st and 2nd harvest than the following harvests.Progenies of A and B had good morphology and high resistance to white mold disease pathogen.The results provided a reference for breeding new morel cultivars by using single ascospre strains.