摘要
以梯棱羊肚菌(Morchella importuna)野生菌株杂交组合A、B以及野生和人工栽培菌株杂交组合C、D子囊果为材料,建立单孢菌株群体,基于其交配型基因挑选145个单孢菌株进行出菇实验,评估单孢菌株产量、出菇整齐度、出菇周期、菇形、抗病性等农艺性状.结果表明:杂交组合B单孢萌发率较高.杂交组合C和D子代单孢菌株平均产量较高;大部分单孢菌株在第1～2次采收时的产量较高.杂交组合A和B子代单孢菌株菇形较好,不易发生病害.研究结果可为利用单孢菌株进行羊肚菌新品种选育提供参考.
Abstract
Morchella importuna is one of the major cultivated morel species in China.However,the commercial cultivation of M.importuna has been troubled by strain degradation,declines in output,and failing to form fruiting bodies.To address these issues,progeny ascocarps from"wild × wild"hybrid combinations ZQW31×ZQW26(A)and ZQW22×ZQW10(B),and"artificial × wild"hybrid combinations 282×ZQW10(C)and 282×ZQW26(D)were used to yield a single-ascospore population.Based on mating type genes,a total of 145 single-ascospore strains were selected for fruiting experiments,and then evaluated for agronomic traits,such as ascospore germination ratio,fruiting yield,fruiting uniformity,fruiting period,ascocarp morphology and disease resistance.The results showed that the germination ratio of B was relatively high.Progenies of C and D had a relatively high yield.Most single ascospore strains showed higher yields at the 1st and 2nd harvest than the following harvests.Progenies of A and B had good morphology and high resistance to white mold disease pathogen.The results provided a reference for breeding new morel cultivars by using single ascospre strains.
国家科技期刊平台
NSTL
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