Biological Characteristics and Domestication of Physisporinus lineatus
A fruiting body of Physiporinus lineatus was collected from Liping County,Qiandongnan Miao and Dong Autonomous Prefecture,Guizhou Province and then subjected to tissue isolation to yield a strain.The strain was studied for biological characteristics through single factor and orthogonal experiments,and then domesticated.The results of the single factor experiments showed that soluble starch was the optimal carbon source for mycelial growth,where the mycelial growth rate reached 5.88 mm·d-1.When yeast extract powder was used as the nitrogen source,the mycelial growth rate reached 5.21 mm·d-1.For cultivation temperature,mycelial growth rate was the greatest at 30 ℃,reaching 6.13 mm·d-1.Addition of CaSO4 in the cultivation medium promoted mycelial growth rate to 5.25 mm·d-1.Preferable C/N ratios were 15∶1 and 45∶1,under which the mycelial growth rate reached 5.17 mm·d-1and 5.02 mm·d-1 respectively.In terms of lighting condition,the mycelial growth rate was the greatest when cultured in the dark,reaching 5.90 mm·d-1.The orthogonal experiment showed that the optimal carbon source,nitrogen source,temperature,and pH for mycelial growth were soluble starch,yeast extract powder,30 ℃,and pH7,respectively.The isolated strain developed fruiting bodies 92-105 d after inoculation,marking the success in the domestication of P.lineatus for the first time.The research provided a reference for the development and utilization of P.lineatus.
Physisporinussingle factormedicinal fungiorthogonal test
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