Optimization of Enzymatic Extraction of Water Soluble Sugars from Ganoderma lucidum Spore Powder and Their Molecular Weight Distribution
The efficiency of enzymatic extraction of water soluble sugars from cell wall residue of Ganoderma lucidum spore powder was optimized through single factor and response surface experiments.The resultant sugars were compared with those extracted by physical methods for molecular weight distribution.The results showed that the optimal enzymatic extraction process was 2％lywallzyme digestion under 1∶10(g∶mL)solid-liquid ratio,40 ℃ and pH6.5 for 5 h.Under the optimal conditions,the water soluble sugar content reached 17.86％.The major component in the resultant water soluble sugars after enzymatic hydrolysis was oligosaccharides.The study provided a reference for effective utilization of G.lucidum spore powder.
Ganoderma lucidum spore powdercell wall water-soluble sugarlywallzymemolecular weight distribution
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