Effects of Pro-glycosylation on Digestion Characteristics and Antioxidant Activity of Digested Products of Lentinula edodes Protein
The effects of pro-glycosylation on digestion characteristics and antioxidant activity of digested products of Lentinula edodes proteins were studied.Proteins were first extracted from fruiting bodies by the alkali dissolution and acid precipitation method,and then glycosylated(G)by glucose and pro-glycosylated(TG)by transglutaminase+glucose,respectively.Proteins without any treatment were the control(C).Through simulated gastrointestinal digestion,C,G,TG were measured for digestibility and hydrolysis degree.The digested products from different groups were measured for free amino acid and peptide contents,DPPH radical scavenging rate,superoxide anion radical scavenging rate,hydroxyl radical scavenging rate,and total antioxidant capacity.The results showed that there was no significant difference between G and TG in digestibility during the course of the simulated digestion.However,their digestibility was significantly lower than that of C at 90,120,240 and 270 min.Compared with C,the hydrolysis degree of G was significantly increased at 60 min of the simulated digestion,and significantly decreased at 90-360 min.The hydrolysis degree of TG was significantly higher than that of C at 30,60,150-240,300 min,and significantly higher than that of G at 60-360 min.The free amino acid content of TG was significantly higher than that of C at 0 and 60-360 min,and significantly higher than that of G at 0,120,300-360 min.The free amino acid content of G was significantly higher than that of C at 60 and 210-360 min.During the entire course of the simulated digestion,the peptide content of G and TG was significantly lower than that of C.At 0 and 300-360 min,the peptide content of TG was significantly higher than that of G.The DPPH scavenging rate of G was significantly higher than that of C during the entire course of the simulated digestion,and also significantly higher than that of TG at 120,150,180,330,360 min.TG also showed a higher DPPH scavenging rate than C at 0,150,210-360 min.The superoxide anion scavenging rate of G at 240-360 min and that of TG at 330 and 360 min were significantly higher than that of C during the simulated intestinal digestion(180-360 min),and the superoxide anion rate of G was also significantly higher than that of TG at 270 min.For hydroxyl radical scavenging rate,both G and TG showed a significantly higher rate than C at all time points of the simulated digestion.The hydroxyl radical scavenging rate of TG was also significantly higher than that of G at 0-180 and 240 min.Both G and TG had stronger total antioxidant capacity than C in the simulated digestion,and G also showed stronger total antioxidant capacity than TG at 0-180 and 240-360 min.