Breeding of Auricularia delicata'Zhongjunzhoumuer-1'
Wild fruiting bodies of Auricularia delicata were collected from Dapingzhang Village,Daxueshan Township,Yongde County,Lincang City,Yunnan Province,and then excellent strains were obtained through tissue isolation and primary screening.Using the strain YD-1 as the control,the best isolated strain was selected through secondary screening,and then compared for differences between the two strains in terms of antagonism,phylogeny,and biological efficiency in pilot and demonstrative field cultivation.The results showed that ZJZME001,ZJZME002 and ZJZME005 were selected during the primary screening for good mycelium vigor,rapid mycelial growth,and relatively high biological efficiency.In the secondary screening,fruiting bodies of YD-1 were yellowish brown,whereas fruiting bodies of ZJZME001,ZJZME002 and ZJZME005 were light yellow.Eventually,ZJZME001 was selected to be the best strain,and it developed obvious antagonistic line against YD-1.Phylogenetic analysis showed that ZJZME001 was clustered in the A.delicata branch.The biological efficiency of ZJZME001 was greater than that of YD-1 in both pilot and demonstrative field production,reaching more than 120％.Compared with YD-1,fruiting bodies of ZJZME001 contained higher levels of zinc,iron,calcium,selenium,protein,total amino acids and vitamin B1.ZJZME001 also contained a lower level of crude fiber than YD-1.On September 25,2023,ZJZME001 passed the identification of non-major crop varieties in Yunnan Province,and was named'Zhongjunzhoumuer-1'.
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