首页|生长期对金耳多糖结构特征及抗炎活性的影响

生长期对金耳多糖结构特征及抗炎活性的影响

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对生长期为25、35、45 d的金耳子实体(Naematelia aurantialba)多糖(分别编号为NAP-1、NAP-2、NAP-3)的得率、多糖含量、糖醛酸含量、分子量分布特征、单糖组成及体外抗炎活性进行比较.结果表明,NAP-3的得率(34.50％)最高,多糖与糖醛酸含量显著高于NAP-1.随着生长期的延长,子实体多糖的重均分子量(weight average molecular weight,Mw)由1.859×106g·mol-1降低至1.347×106g·mol-1.单糖组成分析结果表明,子实体多糖是一种由木糖、甘露糖、葡萄糖醛酸以及少量葡萄糖组成的酸性杂多糖.不同生长期的金耳子实体多糖均能抑制脂多糖诱导引起的RAW264.7巨噬细胞NO释放与炎症因子分泌,与NAP-2、NAP-3相比,NAP-1抑制效果最强.通过Pearson相关性分析,发现金耳子实体多糖的体外抗炎活性与其Mw和甘露糖的摩尔百分比呈正相关,与木糖的摩尔百分比呈负相关.研究结果可为采收获得高抗炎活性的金耳子实体提供技术参数,对金耳健康产品的开发利用提供参考.
Effects of Cultivation Time on Structural Characteristics and Anti-Inflammatory Activity of Polysaccharides from Naematelia aurantialba
Polysaccharides were extracted from fruiting bodies of Naematelia aurantialba at 25 d,35 d and 45 d of cultivation,and were numbered NAP-1,NAP-2 and NAP-3,respectively.NAP-1,NAP-2 and NAP-3 were then compared for yield,polysaccharide content,glucuronic acid content,molecular weight distribution,monosaccharide composition,and anti-inflammatory activity in vitro.The results showed that NAP-3 had the highest yield of 34.50％,and its polysaccharide and glucuronic acid contents were higher than those of NAP-1.As the growth time extended,the weight average molecular weight(Mw)of N.aurantialba polysaccharides(NAPs)decreased from 1.859×106g·mol-1 to 1.347×106g·mol-1.Monosaccharide composition analysis revealed that NAPs were acidic heteropolysaccharides composed of xylose,mannose,glucuronic acid(GlcA),and a small amount of glucose.All three NAPs inhibited NO release and inflammatory factor secretion in RAW264.7 macrophages induced by lipopolysaccharide(LPS).Compared with NAP-2 and NAP-3,the anti-inflammatory efficacy of NAP-1 was better.Pearson's correlation analysis showed that the in vitro anti-inflammatory activity of N.aurantialba was positively correlated with Mw and the molar percentage of mannose,but negatively correlated with the molar percentage of xylose.These findings provided a reference for harvesting N.aurantialba fruiting bodies with high anti-inflammatory activity,and the development of N.aurantialba healthcare products.

Naematelia aurantialbadifferent growth timepolysaccharidemolecular weightmonosaccharide compositionanti-inflammatory activity

刘利平、刘艳芳、孙浩雯、冯杰、杨林雷、曹瑶、李荣春、张劲松

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上海市农业科学院食用菌研究所,农业农村部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点实验室,上海 201403

云南菌视界生物科技有限公司,云南昆明651 708

金耳 不同生长期 多糖 分子量 单糖组成 抗炎活性

现代农业产业技术体系建设项目云南(昆明)张劲松药用真菌专家工作站项目云南省张劲松专家工作站项目

CARS-20YSZJGZZ-2022043202305AF150084

2024

10.16488/j.cnki.1005-9873.2024.03.005

食用菌学报
上海市农业科学院食用菌研究所,上海市农业科学院农业科技信息研究所

食用菌学报

CSTPCD北大核心
影响因子：0.771
ISSN：1005-9873
年,卷(期)：2024.31(3)