广叶绣球菌多糖磷酸化修饰及体外抗氧化和降糖活性
Phosphorylation of Sparassis latifolia Polysaccharides and Their Antioxidant and Hypoglycemic Activities In vitro
崔芳铭 1毕书瑜 1侯江燕 1曹谨玲 2程艳芬 2程菲儿 2云少君 2冯翠萍1
作者信息
- 1. 山西农业大学食品科学与工程学院,山西晋中 030801
- 2. 山西农业大学食品科学与工程学院,山西晋中 030801;黄土高原食用菌山西省重点实验室,山西晋中 030801
- 折叠
摘要
对广叶绣球菌(Sparassis latifolia)多糖(SLPs)进行磷酸化修饰,表征磷酸化SLPs(P-SLPs)结构,分析其体外抗氧化、降糖活性.结果表明:与SLPs比较,P-SLPs的磷酸根质量分数较高,为(8.02±0.48)％;在相同质量浓度时,P-SLPs对DPPH、超氧阴离子、羟基自由基和H2O2的清除率均较高,对α-淀粉酶和α-葡萄糖苷酶的抑制率均较高.研究结果可为广叶绣球菌多糖进一步开发应用提供参考.
Abstract
Sparassis latifolia polysaccharides(SLPs)were modified by phosphorylation,and then characterized for structure,and analyzed for antioxidant and hypoglycemic activities in vitro.The results showed that compared with SLPs,phosphorylated SLPs(P-SLPs)had a higher mass fraction of phosphate ions,which was(8.02±0.48)％.Under the same mass concentration,P-SLPs showed higher abilities on scavenging DPPH radicals,superoxide anion radicals,hydroxyl radicals,and H2O2 than SLPs.The inhibitory activities of P-SLPs on a-amylase and a-glucosidase were also significantly promoted compared with SLPs.The research results provided a reference for further development and application of SLPs.
关键词
广叶绣球菌多糖/磷酸化修饰/抗氧化/降糖活性Key words
Sparassis latifolia polysaccharides/phosphorylation modification/antioxidation/hypoglycemic activity引用本文复制引用
基金项目
山西省现代农业产业技术体系建设项目(2023CYJSTX09)
山西省基础研究计划(202103021224126)
食用菌山西省科技创新重点团队项目(201805D131009)
出版年
2024
国家科技期刊平台
NSTL
万方数据