Comparison of Antioxidant and Antibacterial Activities of Crude Glycoprotein Extracts from Four Edible Fungi In vitro
Using ultrasound-assisted aqueous hydrotropic alcohol precipitation,crude glycoprotein extracts of Russula delica,Boletus edulis,Lyophyllum decastes and Sarcodon imbricatus were obtained respectively.These crude glycoprotein extracts were then determined for molecular weight by SDS-PAGE,chemical composition by Fourier transform infrared absorption spectrometer(FTIR),polysaccharide content by the phenol sulfate method,and protein content by Coomassie blue staining.Their antioxidant activity was assessed in terms of DPPH radical scavenging ability,ABTS radical scavenging ability,and total reducing capacity.These crude glycoprotein extracts were also determined for antibacterial activity against Escherichia coli and Staphylococcus aureus.The results showed that glycoproteins were found in the crude extracts of all four edible fungi,and their molecular weights ranged from 2.5×104 to 6×104.There were typical infrared absorption characteristics of protein and polysaccharide in the crude glycoprotein extracts.Within the experimental range,2.5 mg·mL-1 S.imbricatus crude glucoprotein extract had the highest scavenging rate against ABTS and DPPH free radicals,which were(89.09±4.31)％and(54.95±0.56)％,respectively.The crude glycoprotein extract of S.imbricatus also had the highest total reducing power of 0.99±0.051 at 3 mg·mL-1.For antibacterial activity,the crude glycoprotein extracts from B.edulis and S.imbricatus showed strong activity against E.coli and S.aureus,respectively.
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