Optimization of Liquid Fermentation Conditions and Analysis of Nutritional Components of Leucocalocybe mongolica
Single-factor,Plackett-Burman,steepest ascent and Box-Behnken experiments were used to optimize the carbon source,nitrogen source and inorganic salts in liquid fermentation medium of Leucocalocybe mongolica.Then,single factor experiment was used to optimize the initial pH of the medium,loading volume,inoculum,rotation speed and fermentation temperature.The optimal medium consisted of 35.13 g·L-1 sucrose,16.55 g·L-1 soybean powder and 33 g·L-1 corn steep powder.The optimal initial pH,loading volume,inoculum,temperature,rotation speed and cultivation time were 6.5,75 mL in 250 mL triangular bottles,12.5％,25℃ and 120 r·min-1 for 13 d,respectively.Under these conditions,the mycelial biomass reached 11.37 g·L-1.The contents of crude fat,crude fiber,crude protein and polysaccharide in mycelia were 11.64％,1.32％,27.20％and 34.95％,respectively.
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