Analysis of Lipid-Soluble Components in Ganoderma lucidum Spore Powder and Spore Oil
Lipid-soluble components and chromatographic fingerprints were analyzed for Ganoderma lucidum spore powder treated for different cell-wall breaking time,G.lucidum spore powder of different origins,raw material(cell-wall broken spore powder),intermediates(spore powder after granulation,spore oil of primary extraction,spore oil of secondary extraction)and finished spore oil of G.lucidum spore oil processing,G.lucidum spore oils of different origins,and different vegetable oils.The results showed that the wall-breaking treatment significantly increased the dissolution of lipid-soluble components out of G.lucidum spore powder.Regardless of variations in contents of lipid-soluble components between G.lucidum spore powder samples of different origins,their fingerprint similarity was greater than 0.9,with 18 common peaks.There were 22 common peaks in the fingerprint of raw material(cell wall-broken spore powder),spore powder after granulation,primary extracted spore oil,secondary extracted spore oil and finished spore oil of G.lucidum spore oil processing.Excluding the vitamin E peak in the finished spoil oil,the fingerprint similarity of the raw material,intermediates and the finished spore oil product was greater than 0.99.There were variations in the composition and peak area of the fingerprint peaks of spore oils from different origins,with 23 common peaks and a similarity of 0.490-0.925.The chromatographic peaks of vegetable oil were quite different from those of the spore oils.The results provided a reference for the quality control of G.lucidum spore powder and spore oil products.
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