Transcriptomic Analysis of Genes Related to Lignocellulose Degradation in Lentinula edodes
In order to investigate the molecular mechanism underlying lignocellulose degradation in Lentinula edodes,hybrid progeny ofL.edodes cultivars'Shenxiang 215'and'Huxiang F2'(divided into four groups)were cultured on both PDA medium(control)and sawdust medium.Then mycelia samples of different groups were sequenced for their transcriptiomes.Principle component analysis and correlation analysis were then used to screen and categorize transcriptome data for further analysis.Significantly differentially expressed genes between the PDA and sawdust cultured mycelia samples were identified,and then underwent GO functional enrichment and KEGG metabolic pathway analyses.Nine differentially expressed genes were further selected for qPCR verification.The results showed that culture medium brought about a greater difference than different strains,and the eight different groups of samples were categorized into two groups:PDA group(PA,PB,PC and PE)and sawdust group(MA,MB,MC and ME).Compared with the PDA group,there were 677 differentially expressed genes in the sawdust group,of which 445 genes were up-regulated and 232 genes were down-regulated.Among them,180 of the up-regulated genes and 38 of the down-regulated genes were involved in lignocellulose degradation,suggesting that sawdust significantly induced the expression of genes related to lignocellulose degradation in L.edodes.GO functional enrichment analysis showed that the up-regulated genes were primarily enriched in oxidoreductase activity,hydrolase activity,hydrolase activity(hydrolysis of O-glycosyl compounds),and hydrolase activity(acting on glycosyl bonds).KEGG pathway enrichment analysis showed that the up-regulated genes were significantly enriched in lysosomes,transporters,amino and nucleotide sugar metabolism,arginine and proline metabolism,starch sucrose metabolism,peptidases,and inhibitors.On the other hand,the down-regulated genes were significantly enriched in MAPK signaling-yeast,unsaturated fatty acid synthesis and dioxin degradation.These findings provided a reference for understanding the molecular mechanisms of lignocellulose utilization in L.edodes and breeding strains with high substrate utilization efficiency.
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