Antioxidant and Anti-inflammatory Effects of Engleromyces sinensis Fruiting Body Aqueous Extract in Caenorhabditis elegans
The effects of Engleromyces sinensis fruiting body aqueous extract(ES-AE)against oxidative stress and inflammation induced by tert-butyl hydrogen peroxide(t-BHP)was studied in Caenorhabditis elegans.Six experimental groups were set up as follows:Escherichia coli OP50 as the blank control group,t-BHP group,1.0 mg·mL-1 vitamin C as the positive control group,ES-AE groups at 1.0,10.0 and 20.0 mg·mL-1,six replicates per group.The experimental groups were measured for C.elegans viability,reactive oxygen species(ROS),8-hydroxy-2 deoxyguanosine(8-OHdG),tumor necrosis factor α(TNF-α)and interleukin-1-β(IL-1β).The results showed that the survival rates of nematodes in 1.0,10.0 and 20.0 mg·mL-1 ES-AE groups were significantly increased by 12.85％ ,58.82％ and 61.72％ compared with the t-BHP group,respectively.Compared with the t-BHP group,the ROS levels of 1.0,10.0 and 20.0 mg·mL-1 ES-AE groups were significantly decreased by 9.66％ ,13.65％ and 20.83％ ,respectively;the 8-OHdG of the ES-AE groups were decreased by 5.45％ ,13.64％ and 29.10％ ,respectively;the TNF-α levels of the ES-AE groups were decreased by 25.22％ ,38.51％ and 47.05％ ,respectively;the IL-1 β levels of the ES-AE groups were decreased by 104.14％ ,177.48％ and 190.95％ ,respectively.ES-AE showed a remarkable antioxidant effect on t-BHP-induced oxidative stress in C.elegans in a concentration-dependent manner within the range of 0-20 mg·mL-1.
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