Metabolomic Analysis of Root Exudates of Lactarius hatsudake-Pinus massoniana Mycorrhizal Seedlings
In order to investigate potential substances that promote the growth and mycorrhizal synthesis of Lactarius hatsudake,metabolites in the root exudates of L.hatsudake-Pinus massoniana mycorrhizal seedlings and non-mycorrhizal P.massoniana seedlings were detected by untargeted metabolome(LC-MS/MS)analysis.The results showed that there were 352 differential metabolites in the root system of L.hatsudake-P.massoniana mycorrhizal seedlings under both positive and negative ion modes compared with non-mycorrhizal P.massoniana seedlings.Among these differential metabolites,315 metabolites were significantly up-regulated and 37 metabolites were significantly down-regulated.They were enriched in 28 pathways,such as metabolic pathway,antibiotic biosynthesis,lysine degradation and secondary metabolite biosynthesis.Some differential metabolites,including 2-isopropylmalic acid,phosphoric acid,UDP-N-acetylglucosamine,jasmonic acid,and mevalonic acid were involved in multiple metabolic pathways.These compounds may play an important role in the formation of L.hatsudake-P.massoniana mycorrhiza.Nine significantly different metabolites were selected to study their effects on the mycelial growth of L.hatsudake JH5 and mycorrhizal formation in L.hatsudake-P.massoniana seedlings.D-aspartic acid,DL-arginine,desthiobiotin and(+)-abscisic acid promoted the mycelial growth of L.hatsudake JH5 at 1 mg·L-1,and dethiobiotin,nicotinic acid and(+)-abscisic acid promoted the mycorrhizal formation in L.hatsudake-P.massoniana seedlings at 1 mg·L-1.This study provided a reference for high-efficiency cultivation of L.hatsudake-P.massoniana seedlings.
Lactarius hatsudakemetabolomesymbiosisroot exudates of mycorrhizal seedlings
NETL
NSTL
万方数据
