Development of SSR Molecular Markers Based on Grifola frondosa Genome
Based on 12 Grifola frondosa strains and the published genomic sequence of G.frondosa,192 pairs of SSR primers were designed to study the types and distribution of SSR markers in the G.frondosa,genome and the SSR primers were validated by PCR.The results showed that a total of 666 SSR loci of five SSR types were retrieved.Among them,trinucleotide repeats are the most abundant in G.frondosa,accounting for 52.70％,and the proportion of dinucleotide SSR loci is 32.43％.Repeats of four,five and six bases have a high diversity of repeat motifs,but their quantity was low.Fifteen pairs of polymorphic primers were selected from the validation test,and a total of 55 allele loci were detected.The average Shannon's information index,observed heterozygosity,expected heterozygosity and polymorphism information content of the 15 pairs of SSR primers were 0.817,0.506,0.442 and 0.397,respectively.Using the 15 pairs of SSR primers,the DNA fingerprint coding of G.frondosa was constructed,and the unique 30-digit molecular fingerprint database coding for the 12 G.frondosa strains was obtained,facilitating accurate identification of G.frondosa strains.
万方数据
维普
