Biological Characteristics and Domestication of Lyophyllum pallidofumosum
Wild Lyophyllum pallidofumosum fruiting bodies were collected in Qujing City,Yunnan Province,and a strain named YAASM6292 was obtained through tissue isolation.Then the strain was studied for its optimal carbon source,nitrogen source,inorganic salts,temperature and pH.Then it was domesticated and determined for heavy metal contents in fruiting bodies.The results showed that the optimal carbon source,nitrogen source and pH for YAASM6292 were maltose,peptone,and pH5.0,respectively.Cultivation under 24 ℃ and 26 ℃with the addition of MgSO4 in the medium was preferred by YAASM6292 for its mycelial growth.By using a casing layer,fruiting bodies were obtained for the first time.The heavy metal contents of the domesticated YAASM6292 were below the limits of contaminants in food under National Food Safety Standards.The research results provided a reference for cultivation and utilization of Lyophyllum species.