Effects of Strain and Cultivation Site on Polysaccharide Content and Molecular Weight Distribution Characteristics of Lentinula edodes Fruiting Bodies
Using the phenol-sulfuric acid method,fruiting bodies of 38 Lentinula edodes strains cultivated in Zhumadian City,Henan Province were determined for polysaccharide content.The polysaccharides in different strains were analyzed by high-performance size exclusion chromatography-multi-angle laser light scattering-refractive index detector(HPSEC-MALLS-RI)for molecular weight distribution.According to peak time and number of peaks,the 38 strains were grouped and evaluated for similarity in elution profile and polysaccharide content within and between the groups.Then four randomly selected strains were cultivated both in Suizhou City,Hubei Province,and Zhumadian City,Henan Province,and then compared for polysaccharide content and molecular weight distribution characteristics between fruiting bodies of the same strain cultivated in different sites.The results showed that the polysaccharide contents of the 38 strains were 2.35％-7.82％with an average of 4.98％and a big variation coefficient(28.43％).The samples were divided into six groups(A-F)according to their HPSEC elution profiles.Peak Pl existed in all groups with retention time at 22.3-26.0 min and a weight average molecular weight of 4.259×106-1.039× 107 g·mol-1.Peak P2 also existed in all groups with a weight average molecular weight greater than 106 g·mol-1,but there were variations in retention time between different groups.Peak P3 only existed in groups D,E and F with a weight average molecular weight of 2.415×105-5.235 × 106 g·mol-1,and its retention time was over 30 min.The similarity of the elution profiles within groups was 0.915-0.997,and the similarity between different groups was less than 0.7 except group A and group B,which was 0.847.There was no correlation between polysaccharide content and molecular weight distribution profile.For the same strain,there were differences in both composition and content of polysaccharides between fruiting bodies cultivated in different sites.These results provided a reference for selection of superior fruiting bodies in preparation of polysaccharides from L.edodes.
Different strainsLentinula edodespolysaccharide contentmolecular weight distribution
万方数据
维普
