The nutrient composition of Tuber sinense was determined,and then its fruiting body powder was added to the basic mouse feed at 1％(test group Ⅰ,1％T.sinense+99％basic diet)and 5％(test group Ⅱ,5％T.sinense+95％basic diet),respectively.Mice fed with the basic feed served as the normal control group.At 12 d,24 d and 36 d,mice in different groups were measured for spleen index,thymus index,leukocyte count,percentages of five types of leukocytes,the percentage of T lymphocytes,and serum IgG level.At 36 d,relative expression levels of TNF-α,IL-1βand IL-6 in spleen were also determined.The results showed that compared with the control group,the spleen index of group Ⅱ was significantly decreased at 36 d;the number of leukocytes in group Ⅰ was significantly increased at 12 d,24 d and 36 d;the percentage of monocytes in group Ⅱ was significantly increased at 24 d and 36 d;both group Ⅰ andⅡ had significantly increases in percentages of neutrophils and lymphocytes(24 d and 36 d for group Ⅰ,and 12 d,24 d and 36 d for group Ⅱ);the percentage of T lymphocytes in group Ⅱ was significantly increased at 24 d and 36 d;the serum IgG content in group Ⅰ was significantly increased at 36 d,and that of group Ⅱ was significantly increased at 12 d,24 d and 36 d;the relative TNF-α expression in spleen was significantly increased in both group Ⅰ and Ⅱ at 36 d.The results provided a reference for the development and utilization of T.sinense.
万方数据
维普
