Identification and Analysis of Lignin-Responsive Transcription Factors in Ganoderma lucidum
Lignin-responsive transcription factors in Ganoderma lucidum were identified through comparison of the transcriptome data of G.lucidum GL0102 mycelia cultured with glucose as the carbon source versus lignin as the carbon source.Differentially expressed transcription factors(TFs)were identified,validated,and then determined for expression levels under different agricultural and forestry wastes(bagasse,rice straw,wheat straw,oak sawdust,spruce sawdust,and pine sawdust).Three interference strains of the differential transcription factor chr8g0158731 were constructed,and then determined for mycelial growth rate under different carbon sources and lignin utilization.The results showed that there were 26 up-regulated and 40 down-regulated TFs in the transcriptome of mycelia cultured on lignin compared with that of mycelia cultured on glucose.When cultured on the agricultural and forestry wastes,seven of the up-regulated TFs showed a high expression level in mycelia,and six of the down-regulated TFs showed a low expression level.When cultured with glucose as the carbon source,the mycelial growth rates of the three interference strains were not significantly different from that of the wild-type strain.When cultured on lignin and the six agricultural and forestry wastes,the mycelial growth rates of the three interference strains were greater than that of the wild-type strain,and the expression level of chr8g0158731 in the the three interference strains was lower than that in the wild-type strain.When cultured with lignin as the carbon source,all three interference strains degraded lignin to a greater extent than the wild-type strain.The results provided candidate genes for subsequent lignin degradation studies.
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