Fruiting Characteristics after Pairing of Single Mating Type Strains of Cordyceps militaris
Cordyceps militaris strains D1,D2,S1,S2 and S3 were cultivated and then determined for their mating type.Monoconidial strains were isolated from the five strains and determined for mating type.Paired crossing between different mating types of monoconidial strains isolated from S1,S2 and S3 were carried out.The MAT1-2 type monoconidial strains of S1,S2 and S3 were also crossed with monoconidial strains of D1 and D2.Superior hybrid strains were then screened out of the crossing progenies.The results showed that D1,D2,S1,S2 and S3 fruited stably.Fruiting bodies of D1 and D2 were smooth,lack of perithecium,and exclusively MAT1-1 mating type.On the other hand,fruiting bodies of S1,S2 and S3 had abundant perithecium on the surface,and contained either MAT1-1 or MAT1-2 mating type gene.All paired-crossing progenies developed perithecium on the surface of their fruiting bodies,and produced ascospores.After screening for agronomic traits,superior strains D1S1-38,D2S1-15,D2S1-43(MAT1-2),D2S1-35 and D2S1-44(MAT1-1)were selected.These findings provided a reference for genetic breeding of C.militaris.
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