摘要
栽培蛹虫草(Cordyceps militaris)菌株D1、D2、S1、S2、S3,鉴定其交配型;分别从5个菌株分离单分生孢子菌株,鉴定其交配型;开展菌株S1、S2、S3的不同交配型单分生孢子菌株配对栽培实验以及菌株D1、D2的单分生孢子菌株与菌株S1、S2、S3的单分生孢子菌株(MAT1-2型)配对栽培实验;从杂交菌株子囊孢子菌株中筛选优良菌株.结果表明:供试的5个蛹虫草菌株均具有稳定出菇能力,菌株D1和D2子实体表面光滑,无子囊壳,只含有MAT1-1基因;菌株S1、S2、S3子实体表面有丰富的子囊壳,含有MAT1-1和MAT1-2基因.菌株S1、S2、S3的不同交配型单分生孢子菌株配对栽培,菌株D1、D2的单分生孢子菌株与菌株S1、S2、S3的单分生孢子菌株(MAT1-2型)配对栽培后形成的子实体表面均形成子囊壳,产生子囊孢子.筛选获得优良菌株D1S1-38、D2S1-15、D2S1-43(MAT1-2 型)和 D2S1-35、D2S1-44(MAT1-1 型).研究结果可为蛹虫草遗传育种研究提供参考.
Abstract
Cordyceps militaris strains D1,D2,S1,S2 and S3 were cultivated and then determined for their mating type.Monoconidial strains were isolated from the five strains and determined for mating type.Paired crossing between different mating types of monoconidial strains isolated from S1,S2 and S3 were carried out.The MAT1-2 type monoconidial strains of S1,S2 and S3 were also crossed with monoconidial strains of D1 and D2.Superior hybrid strains were then screened out of the crossing progenies.The results showed that D1,D2,S1,S2 and S3 fruited stably.Fruiting bodies of D1 and D2 were smooth,lack of perithecium,and exclusively MAT1-1 mating type.On the other hand,fruiting bodies of S1,S2 and S3 had abundant perithecium on the surface,and contained either MAT1-1 or MAT1-2 mating type gene.All paired-crossing progenies developed perithecium on the surface of their fruiting bodies,and produced ascospores.After screening for agronomic traits,superior strains D1S1-38,D2S1-15,D2S1-43(MAT1-2),D2S1-35 and D2S1-44(MAT1-1)were selected.These findings provided a reference for genetic breeding of C.militaris.
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