摘要
分别采用黑暗、蓝光和红光处理糙皮侧耳(Pleurotus ostreatus)菌丝,测定菌丝生长指标、木质纤维素降解酶(漆酶、纤维素酶、木聚糖酶)活性及木质纤维素降解酶基因[漆酶基因(LAC)、外切葡聚糖酶基因(EXO)和内切葡聚糖酶基因(END)、葡萄糖苷酶基因(GLU)]相对表达量.结果表明:在实验范围内,与黑暗、蓝光相比,红光处理的菌丝生长速度最快、生物量最高.培养3 d时,黑暗处理的菌丝漆酶、纤维素酶、木聚糖酶活性均最高,LAC、END的相对表达量均最高.培养7 d时,红光处理的菌丝漆酶、纤维素酶活性最高,EXO、END相对表达量最高;蓝光处理的菌丝木聚糖酶活性最高,GLU相对表达量最高.培养10 d时,黑暗处理的漆酶活性最高,GLU相对表达量最高;黑暗和红光处理的纤维素酶活性、木聚糖酶活性显著高于蓝光;红光处理的LAC和END相对表达量最高.
Abstract
Mycelia of Pleurotus ostreatus were cultivated under darkness,blue light,and red light,respectively.Then mycelia of different light quality groups were measured for growth parameters,activities of lignocellulose-degrading enzymes,i.e.,laccase,cellulase,and xylanase,and relative expression of lignocellulose-degrading enzyme genes,including laccase gene(LAC),exoglucanase gene(EXO),endoglucanase gene(END),and glucosidase gene(GLU).The results showed that red light resulted in the highest mycelial growth rate and biomass.After cultivation for 3 d,darkness resulted in the highest activities of laccase,cellulase and xylanase,and the highest expression levels of LAC and END.After cultivation for 7 d,red light resulted in the highest activities of laccase and cellulase,and the highest expression levels of EXO and END,and mycelia cultivated under blue light showed the highest xylanase activity and GLU expression.After cultivation for 10 d,mycelia under darkness showed the highest laccase activity and the highest expression level of GLU;the activities of cellulase and xylanase under darkness and red light treatments were significantly higher than those under blue light treatment;red light resulted in the highest expression levels of LAC and END.
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