Effects of Light Quality on Mycelial Growth and Extracellular Enzyme Activity of Pleurotus ostreatus
Mycelia of Pleurotus ostreatus were cultivated under darkness,blue light,and red light,respectively.Then mycelia of different light quality groups were measured for growth parameters,activities of lignocellulose-degrading enzymes,i.e.,laccase,cellulase,and xylanase,and relative expression of lignocellulose-degrading enzyme genes,including laccase gene(LAC),exoglucanase gene(EXO),endoglucanase gene(END),and glucosidase gene(GLU).The results showed that red light resulted in the highest mycelial growth rate and biomass.After cultivation for 3 d,darkness resulted in the highest activities of laccase,cellulase and xylanase,and the highest expression levels of LAC and END.After cultivation for 7 d,red light resulted in the highest activities of laccase and cellulase,and the highest expression levels of EXO and END,and mycelia cultivated under blue light showed the highest xylanase activity and GLU expression.After cultivation for 10 d,mycelia under darkness showed the highest laccase activity and the highest expression level of GLU;the activities of cellulase and xylanase under darkness and red light treatments were significantly higher than those under blue light treatment;red light resulted in the highest expression levels of LAC and END.
NETL
NSTL
万方数据
