Ameliorating Effect of Sparassis latifolia Polysaccharide on Hippocampal Injury in Lead-Exposed Mice
Kunming mice were randomly divided into blank control(NC)group,lead exposure(PB)group,Sparassis latifolia polysaccharide(SLP)group,and lead exposure(Co-PB)group co-housed with SLP treatment(Co-SLP)group.Except NC group,mice in the rest groups were given 1 g·L-1lead acetate solution as drinking water,and mice in SLP and Co-SLP groups were also administered 400 mg·kg-1SLP by gavage.After 8 weeks,mice in different groups were measured for lead content in hippocampus by graphite furnace atomic absorption spectrometry,observed for hippocampal tissue structure by hematoxylin-eosin staining,determined for contents of malondialdehyde(MDA)and glutathione(GSH),and determined for activities of total superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPx)in hippocampus.Real-time quantitative PCR was used to detect relative expression of inflammation-related genes IL-4,IL-6,IL-10,IL-1β,TNF-α,TGF-β,and autophagy related genes Beclin-1,LC3-I,LC3-Ⅱ,Lamp2,P62 and Atg5 in hippocampus.The results showed that the hippocampal lead contents of SLP,Co-SLP and Co-PB were significantly lower than that of PB.There were significant increases of CAT activity and GPx activity in SLP mice and Co-SLP mice compared with PB mice.The SOD activity and GSH content of SLP,Co-SLP and Co-PB groups were also increased significantly.On the other hand,the contents of MDA in SLP,Co-SLP and Co-PB groups decreased significantly compared with that in PB group.Compared with PB mice,mice treated with SLP showed significantly decreased expression of IL-1β,IL-6 TNF-α,LC3Ⅱ,Lamp2,Beclin1,LC3-I and Atg5,and significantly increased expression of IL-4,TGF-β and IL-10.Compared with PB mice,Co-SLP mice showed significantly decreased expression of IL-6,TNF-a,LC3 Ⅱ,Lamp2,Beclin1,LC3-I and Atg5,and significantly increased expression of IL-4,TGF-β,IL-10 and P62.Compared with PB mice,Co-PB mice showed significantly decreased expression of TNF-α,LC3-I and Atg5,and significantly increased expression of TGF-β and IL-10.The results provided a reference for ameliorating lead injury by S.latifolia polysaccharide.
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