Ethyl Acetate Extract of Inonotus obliquus Reduced Uric Acid in Hyperuricemia Model Mice
The uric acid lowering effect of ethyl acetate extract from Inonotus obliquus on hyperuricemic mice was studied.Mice were randomly divided into the following groups:normal group,hyperuricemia model group,allopurinol group(5 mg·kg-1,positive BA),benzbromarone group(7.8 mg·kg-1,positive BB),low-dose I.obliquus ethyl acetate extract(IO-E)group at 50 mg·kg-1,and high-dose I.obliquus ethyl acetate extract group at 100 mg·kg-1.Except the normal group,all the other groups of mice were administered potassium oxyzinate(300 mg·kg-1)intraperitoneally and hypoxanthine(500 mg·kg-1)by gavage for 14 consecutive days to establish a hyperuricemia model.After successful modeling,mice in different groups were administered corresponding interventions once a day for 28 consecutive days.One hour after the last administration,mice in different groups were measured for levels of uric acid,urea nitrogen,creatinine,xanthine oxidase,and adenosine deaminase in the blood by enzyme colorimetry,calculated for kidney and liver indices,observed for pathological changes in the kidneys and livers by hematoxylin-eosin staining,determined for URAT1 and GLUT9 protein contents in the kidneys by enzyme linked immunosorbent assay,observed for the localization of URAT1 and GLUT9 in the kidney tissues by immunohistochemical staining,and determined for the expression of URAT1 and GLUT9 by immunoblotting.Compared with the model group,mice in positive BA,positive BB and I.obliquus ethyl acetate extract groups showed ameliorated hyperuricemic symptoms,including decreased kidney and liver indices,decreased levels of blood uric acid,urea nitrogen and creatinine,decreased xanthine oxidase and adenosine deaminase activity,and ameliorated pathological changes in the kidney and liver tissues.The levels of URAT1 and GLUT9 in the kidneys of BA,BB and IO-E treated mice were significantly reduced,and the xanthine oxidase activity in the livers of these groups was also significantly reduced.Immunohistochemical staining of URAT1 and GLUT showed that URAT1 was primarily expressed in the cytoplasm of renal tubular epithelial cells,and GLUT9 was primarily expressed in the lateral membrane and cytoplasm of renal tubular epithelial cells.Compared with the model,BA,BB and IO-E resulted in significant decreases in average optical density(AOD)value and down-regulated expression of URAT1 and GLUT in kidney.Ethyl acetate extract of I.obliquus showed a uric acid lowering effect and effectively ameliorated kidney and liver damage caused by hyperuricemia.
NETL
NSTL
万方数据
