Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Detection of Ergosterol Peroxide in Edible Fungi and Their Processed Products
Using ultra-high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),a method was established for measuring ergosterol peroxide(EP)content in edible fungi and their processed products through optimizing chromatographic conditions,mass spectrometric parameters,extraction solvents,extraction time,and temperature.The method was validated through matrix effect assessment,spike-and-recovery testing,and evaluation of repeatability and reproducibility.The established method was used to measure EP content in 31 samples of edible fungi and processed products.The results showed that the chromatographic conditions were using a C18 chromatographic column and isocratic elution with 0.1％ formic acid in methanol and 0.1％ formic acid in water at a volumetric ratio of 92∶8.Aacetonitrile was used as the extraction solvent for ultrasonic extraction at 30 ℃ for 30 min.The quantitative ion transition was m/z 429.0/191.0.The optimal extraction conditions were There was good linearity in the range of 5 to 2 000 ng·mL-1with minimal matrix effects.The EP detection limit for fresh and pickled edible fungi products was 0.020 mg·kg-1,and that of dried and ready-to-eat edible fungi products was 0.040 mg·kg-1.The spike recovery rates were 78.4％ -111.5％ ,which comply with the national standard of 60％ -120％ in GB 2763-2021.The intraday variation coefficients and interday variation coefficients were 0.3％ -4.8％ ,and 0.4％ -2.0％ ,respectively.Fresh L.edodes had a relatively high EP content.
Edible mushroomergosterol peroxideultra-high-performance liquid chromatography-tandem mass spectrometryquantitation
NETL
NSTL
万方数据
