摘要
目的:探究甲羟戊酸(MEV)及氟伐他汀钠(FS)对舌鳞癌细胞的抑制作用.方法:不同浓度的FS和MEV分别或联合处理舌鳞癌细胞HSC-4.用CCK-8、流式细胞术、划痕实验、免疫荧光和Western blot技术分别检测细胞增殖、凋亡、迁移以及RAS同源基因家族成员A(RHOA)、组织因子(TF)和BAX蛋白的表达情况.结果:FS抑制HSC-4细胞的增殖和迁移,降低细胞内RHOA和TF的表达,促进BAX表达.MEV逆转FS对HSC-4细胞的抑制作用,促进细胞内RHOA和TF的表达(P＜0.05).结论:MEV体外逆转FS对舌鳞癌细胞HSC-4的抑制作用.
Abstract
Objective:To investigate mevalonic acid(MEV)on reversing the antitumor effects of fluvastatin sodium(FS)on tongue squamous cell carcinoma(TSCC)cells.Methods:TSCC HSC-4 cells were treated with different concentrations of FS and MEV re-spectively or in combination.Cell counting kit-8,flow cytometry,wound healing,immunofluorescence and western blotting assays were conducted to examine cell proliferation,apoptosis and migration capacities,as well as the expression levels of Ras homolog family member A(RHOA),tissue factor(TF)and B-cell lymphoma-2-associated X(BAX)proteins,respectively.Results:FS inhibited HSC-4 cell proliferation and migration,decreased the expression of RHOA and TF,and increased BAX expression.MEV reversed the above antitumor effects of FS and promoted intracellular RHOA and TF expression.Conclusion:MEV reverses the antitumor effects of FS on HSC-4 cells in vitro.
基金项目
云南省卫生科技计划(2018NS212)
云南省卫生健康委医学领军人才项目(L-2018010)
昆明医科大学研究生创新基金(2019S096)
维普
万方数据