微流控芯片制备载镁水凝胶核-壳微球的体外成骨活性研究
Osteogenic activity of magnesium loaded hydrogel core-shell microspheres by microfluidic chip in vitro
李龙 1梁海 1刘斌 2吴健 1朱洁 1林正捷3
作者信息
- 1. 518067,深圳市前海蛇口自贸区医院口腔科
- 2. 口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,空军军医大学第三附属医院口腔生物学教研室
- 3. 深圳市前海蛇口自贸区医院3D打印与再生医学创新中心
- 折叠
摘要
目的:探索载镁水凝胶微球的在体外细胞实验中的成骨活性.方法:设计和搭载微流控芯片装置,并利用该装置载镁水凝胶核-壳微球精准调控镁离子在体外稳定释放,系统研究可控镁离子释放促进成骨活性机制.结果:微流控芯片制备出的载镁水凝胶微球(PLGA/MgO-alginate微球)呈现单分散性和特殊的核-壳结构调控镁离子在体外稳定释放的浓度稳定在50 ppm.不仅能显著增强MC3T3-E1前成骨细胞的活性和增殖能力,还可以改善其成骨分化和矿化能力,并增强了 ALP活性.结论:载镁水凝胶核-壳微球能显著提高MC3T3-E1前成骨细胞的成骨活性.
Abstract
Objective:To explore the osteogenic activity of in vitro cultured preosteoblasts treated by magnesium loaded hydrogel core-shell microspheres.Methods:A microfluidics chip device was designed and made to fabricate magnesium loaded hydrogel core-shell microspheres to precisely control the stable release of magnesium ions in vitro,the mechanism of controllable magnesium ion release in the promotion of osteogenic activity of MC3T3-E1 preosteoblasts was systematically investigated.Results:The magnesium loaded hydro-gel microspheres(PLGA/MgO-alginate microspheres)prepared by the microfluidics chip exhibited to be monodisperse with special core-shell structure and could control the stable release of magnesium ions at a concentration of 50 ppm in vitro.The in vitro study showed that it significantly enhanced the activity and proliferation ability of MC3T3-E1 preosteoblasts,promoted their osteogenic differ-entiation and mineralization ability,and increased ALP activity.Conclusion:Magnesium loaded hydrogel core-shell microspheres can promote the osteogenic activity of MC3T3-E1 osteoblasts in vitro.
关键词
微流控芯片/水凝胶/成骨活性/骨再生/镁离子Key words
Microflluidic chip/Hydrogels/Osteogenic activity/Bone regeneration/Magnesium ions引用本文复制引用
基金项目
广东省深圳市南山区卫生科技项目(NS2021064)
深圳市科技计划(JCYJ20220530151608019)
广东省基础与应用基础研究基金(2022A1515011815)
广东省基础与应用基础研究基金(2023A1515220232)
出版年
2024
NETL
NSTL
万方数据