The effects of HDAC3-Nrf2 signaling pathway on the adipogenesis of condylar chondrocytes in rats with temporomandibular joint osteoarthritis
Objective:To observe the effects of histone deacetylase 3(HDAC3)-nuclear factor-erythroid 2-related factor 2(Nrf2)signaling pathway on the adipogenesis of condylar chondrocytes in rats with temporomandibular joint(TMJ)osteoarthritis(OA).Meth-ods:60 6-week-old female SD rats were randomly divided into 10 groups(n=6):control,unilateral anterior crossbite(UAC)stimula-tion,UAC with HDAC3 inhibitor RGFP966 injection(UAC+RGFP966)and UAC with Nrf2 agonist Bardoxolone injection(UAC+Bard-oxolone)groups.The animals were sacrificed at 4,8 and 12 weeks after set up of the experiment,and TMJ condylar cartilage was taken for immunohistochemical(IHC)staining of HDAC3,Nrf2 and Adiponection.Results:Compared with the control group,the de-generation of TMJ condylar cartilage in the UAC group was obvious,the positive cell rates of HDAC3 and Adiponectin were increased(P＜0.05),while the positive cell rate of Nrf2 decreased(P＜0.05).Compared with UAC group,after injection of RGFP966 and Bardox-olone,the positive cell rates of HDAC3 and Adiponectin in TMJ condylar cartilage of rats were decreased(P＜0.05),the positive cell rate of Nrf2 increased(P＜0.05),and the degree of cartilage degeneration was reduced.Conclusion:UAC promotes the adipogenesis of TMJ OA condylar chondrocytes through HDAC3-Nrf2 signaling pathway.Local drug injection intervention of HDAC3-Nrf2 signaling can inhibit the adipogenesis and cartilage degeneration of TMJ OA condylar chondrocytes.
NETL
NSTL
万方数据
