The effects of gelatin or polydopamine-modified poly caprolactone electrospun membranes on the biological behavior and osteogenic differentiation of MC3T3-E1 cells
Objective:To compare the effects of gelatin(Gel)and polydopamine(PDA)modification of polycaprolactone(PCL)on the biological behaviour and osteogenic function of osteoblasts.Methods:PCL electrospun membranes were prepared by electrostatic spinning technique,PCL surface was modified by Gel and PDA respectively as G/PCL and D/PCL with chemical self-assembly tech-nique,and the physicochemical properties of the electrospun membranes were characterized by scanning electron microscopy(SEM),Fourier infrared spectroscopy(FTIR),X-ray photoelectron spectroscope(XPS)and contact angle measurement.The MC3T3-E1 cell adhesion morphology was observed by SEM,immunofluorescence staining followed by confocal microscopy(CLSM),cell proliferation at 1,3 and 5 d was tested by CCK-8 assay,alkaline phosphatase(ALP)staining,alizarin red staining and qRT-PCR were used to detect osteogenic gene expression of the cells.Results:A coating of PDA particles was observed on the surface of D/PCL film.FTIR and XPS showed that the characteristic peaks of Gel and PDA,and there was no obvious droplets on the surface of G/PCL and D/PCL ob-served by contact angle test.Cell density of G/PCL group was higher,the adhesion morphology was good and pseudopods were obvi-ous.CCK-8 assay showed the highest proliferation of the cells on G/PCL(P＜0.05).ALP and alizarin red staining of the cells were stronger in D/PCL group than in the other 2 groups.qRT-PCR results showed that the mRNA expression of ALP,COL-1,RUNX2 and OCN was higher in the D/PCL group than in the other 2 groups.Conclusion:Both Gel and PDA modification can enhance the cell adhesion,proliferation and osteogenic properties of PCL scaffolds,Gel modification may induce a more pronounced proliferative effect and PDA modification more pronounced osteogenic effect.
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