口腔鳞状细胞癌中ANO1的表达及临床意义
Expression and clinical significance of ANO1 in oral squamous cell carcinoma
闫红娟 1马晓平 2李曼 3仵楠 1唐小雪 1陈敏 1徐江1
作者信息
- 1. 832000,石河子大学第一附属医院口腔科
- 2. 832000,石河子大学第一附属医院肿瘤内科
- 3. 832000,石河子大学第一附属医院病理科
- 折叠
摘要
目的:探讨ANO1在口腔鳞状细胞癌(OSCC)中的表达及预后价值.方法:分别采用免疫组织化学法(n=163)和实时荧光定量PCR(n=42)检测ANO1蛋白和mRNA在OSCC及癌旁正常组织中的表达水平,分析ANO1的表达与OSCC患者临床病理特征(n=163)和预后(n=93)的关系,并与TCGA数据库样本分析结果进行比较.结果:免疫组化和实时荧光定量PCR结果证实ANO1在OSCC中高表达(P＜0.05),与TCGA数据库分析结果一致.ANO1蛋白表达与T分期、淋巴结转移、TNM分期、预后不良密切相关(P＜0.05).Cox回归分析结果显示,ANO1蛋白表达(P=0.002)和分化程度(P=0.034)是影响OSCC患者预后的独立危险因素.结论:ANO1在OSCC组织中高表达,且与患者不良预后相关,可作为肿瘤患者不良预后的新型生物标志物.
Abstract
Objective:To investigate the expression and prognostic value of ANO1 in oral squamous cell carcinoma(OSCC)tissues.Methods:Immunohistochemistry(IHC,n=163)and Quantitative real-time PCR(qRT-PCR,n=42)were employed to detect the expression level of ANO1 protein and mRNA in OSCC tissues and paracancerous normal tissues.The relationship between ANO1 ex-pression and clinicopathological features(n=163)and prognosis(n=93)of the patients were analyzed,and the results were compared with those in TCGA database.Results:IHC and qRT-PCR confirmed that ANO1 was highly expressed in OSCC(P＜0.05),which was consistent with the results of the TCGA database.Cox regression analysis showed that ANO1 expression was significantly correlated with T stage,lymph node metastasis and TNM stage and poor prognosis(P＜0.05).By Cox regression analysis,ANO1 overexpression(P=0.002)and differentiation degree(P=0.034)were independent prognostic factors.Conclusion:ANO1 is highly expressed in OSCC and is correlated with poor prognosis,which can be used as a novel biomarker for poor prognosis of OSCC patients.
关键词
口腔鳞状细胞癌/ANO1/免疫组织化学/实时荧光定量PCR/预后Key words
Oral squamous cell carcinoma/A NO 1/Immunohistochemistry/qRT-PCR/Prognosis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据