可注射釉基质蛋白水凝胶制备及其性能研究
Preparation and characterization of an injectable enamel matrix protein hydrogel
王春宝 1曾子瑜 2全雅琦 2王忠山3
作者信息
- 1. 336000,宜春学院化学与生物工程学院
- 2. 空军军医大学基础医学院学员三大队十二队
- 3. 口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔医学重点实验室,第四军医大学口腔医院口腔修复科
- 折叠
摘要
目的:构建一种负载釉基质蛋白(EMP)的泊洛沙姆(Poloxamer)可注射温敏水凝胶,研究其性能.方法:提取6月龄幼猪牙胚EMP,制备空白凝胶以及负载釉基质蛋白(2mg/mL)的凝胶(P407∶P188分别为23∶8和19∶1),采用试管倒置法测定胶凝温度,超微量蛋白定量法测定优选组方的EMP释放性能.结果:负载2mg/mL EMP,P407∶P188为23∶8的泊洛沙姆可注射温敏水凝胶(编号FP1)胶凝温度为33℃,其释放性能符合Higuchi方程,适合临床应用.结论:构建了可缓释EMP的泊洛沙姆可注射温敏水凝胶,该体系具有合适的相转变温度和控释效能.
Abstract
Objective:To prepare an enamel matrix protein(EMP)loaded poloxamer thermosensitive injectable hydrogel,and to study its characteristics.Methods:EMP was extracted from a 6-month old porcine and identified by SDS-PAGE electrophoresis.EMP at 2 mg/mL was loaded into gels poloxamer with P407∶P188 of 23∶8 and 19∶1 respectively.The gelling temperature of blank gel and EMP gel was detected by an inverted tube method.The release performance of selected EMP-loaded gel was detected by an Ultra-micro spectrophotometer.Results:A hydrogel(code number:FP1)with formula of 2 mg/mL EMP and 23∶8 of P407∶P188 possessed gel-ling temperature of 33 ℃,and the release behavior of FP1 conformed to the Higuchi equation.Conclusion:A poloxamer thermosensitive injectable hydrogel was successfully fabricated,this formulation possesses adaptive phase inversion temperature and controlled release ef-ficiency.
关键词
泊洛沙姆/温敏可注射水凝胶/釉基质蛋白/释放性能Key words
Poloxamer/Thermosensitive injectable hydrogel/Enamel matrix protein/Release performance引用本文复制引用
基金项目
国家自然科学基金青年项目(81700930)
福建省自然科学基金青年创新项目(2019J05117)
江西省教育厅科研项目(GJJ190840)
陕西省重点研发计划一般项目(2021SF-105)
出版年
2024
NETL
NSTL
万方数据