MCP-1/CCR2轴介导NF-κB信号途径对致炎因素刺激的牙髓干细胞迁移的影响
The influence of MCP-1/CCR2 axis mediated NF-κB signal pathway on the migration of dental pulp stem cells steamulated by inflamation factor
李伟 1彭建安 2杨赣军 2胡红梅2
作者信息
- 1. 343000,井冈山大学医学部口腔系
- 2. 井冈山大学医学部组织学与解剖学教研室
- 折叠
摘要
目的:研究MCP-1/CCR2轴在介导NF-KB信号途径影响致炎因素刺激的牙髓干细胞(DPSCs)迁移的分子机制.方法:取SD大鼠进行DPSCs原代培养,流式细胞仪鉴定后建立DPSCs细胞LPS炎症模型,Western blot和Q-PCR检测各组DPSCs中MCP-1、CCR2表达,NF-κB通路激活剂TNF-α和NF-κB通路抑制剂BMS-345541作用炎症DPSCs,免疫荧光检测炎症DP-SCs 中P65的活化水平,划痕实验检测DPSCs迁移能力.结果:LPS诱导处理的DPSCs组显示MCP-1、CCR2基因积分光密度高于正常组,TNF-α处理DPSCs组显示MCP-1、CCR2基因积分光密度高于LPS诱导处理组和NF-κB通路抑制剂BMS-345541处理组,TNF-α处理DPSCs能促进划痕面积的愈合,且比NF-κB通路抑制剂BMS-345541处理组效果更明显.结论:在炎症状态下DPSCs表达MCP-1、CCR2基因较高,NF-κB信号途径的激活可以加强这一表达,促进DPSCs的横向迁移能力.
Abstract
Objective:To explore the molecular mechanism of NF-κB signaling pathway mediated by MCP-1/CCR2 axis in the migra-tion of dental pulp stem cells(DPSCs)stemulated by inflamation factor.Methods:DPSCs of SD rats were in vitro cultured,and identified by flow cytometry.The inflammation model of DPSCs was established by LPS treatment,Western blot and Q-PCR were used to detect the expression of MCP-1 and CCR2 in DPSCs of the groups.The inflamatory DPSCs were treated by NF-κB pathway activator TNF-α and NF-κB pathway inhibitor BMS-345541 respectively,the activation of P65 in inflammatory DPSCs was detected by immuno-fluorescence.Scratch experiment was used to detect the migration ability of DPSCs.Results:Higher expression of MCP-1 and CCR2 genes was observed in LPS-induced DPSCs than in the controls,TNF-α treated DPSCs showed higher expression of MCP-1 and CCR2 genes than the LPS tread and the NF-κB pathway inhibitor BMS-345541 treated cells.TNF-α treated DPSCs showed higher scratch healing effect than LPS treated and BMS-345541 treated cells.Conclusion:The expression of MCP-1 and CCR2 genes in DPSCs is higher in inflammatory state,and the activation of NF-κB signaling pathway can enhance this expression,which can ultimately promote the migration ability of DPSCs in inflammatory state.
关键词
MCP-1/CCR2轴/信号途径/牙髓干细胞/迁移Key words
MCP-1/CCR2 axis/Signaling pathway/Dental pulp stem cells/Migration引用本文复制引用
基金项目
江西省自然科学基金(20202BAB206037)
江西省卫健委科技计划项目(202130707)
出版年
2024
NETL
NSTL
万方数据