二甲双胍介导高糖高脂环境下大鼠颌骨BMSCs成骨分化的机制研究
The mechanism of metformin-mediated osteogenic differentiation of rat jaw BMSCs under high-glucose and high-fat environment
齐东杰 1周鑫磊 1嵇步云 2林家婷2
作者信息
- 1. 241002 芜湖,皖南医学院口腔医学院
- 2. 皖南医学院第一附属医院弋矶山医院口腔科
- 折叠
摘要
目的:探讨二甲双胍介导高糖高脂环境下大鼠颌骨骨髓间充质细胞(BMSCs)成骨分化机制.方法:体外分离并培养SD大鼠(雄性)下颌骨BMSCs,分为对照组(C组),高糖高脂组(H组),二甲双胍刺激的正常组(CM组),二甲双胍刺激的高糖高脂组(HM组).EDU染色检测探究二甲双胍对BMSCs增殖的影响;BMSCs经诱导后,用碱性磷酸酶染色、茜素红染色和油红O染色检测细胞成骨分化和成脂分化能力;用Western blot和免疫荧光染色检测BMSCs成骨分化因子runt-相关转录因子2(Runx2)、骨钙素(OCN)的蛋白表达量.结果:HM组较H组比较,细胞增殖能力增强,Runx2、OCN表达水平升高(P＜0.05),成骨分化能力显著提高,成脂分化能力显著下降.结论:二甲双胍可诱导BMSCs表达Runx2和OCN,促进体外培养的高糖高脂环境下大鼠颌骨BMSCs增殖与成骨分化.
Abstract
Objective:To explore the mechanism of metformin on osteogenic differentiation of rat jaw bone marrow mesenchymal cells(BMSCs)under high sugar and high fat environment.Methods:BMSCs of male SD rats were isolated and cultured in vitro and divided into 4 groups:Control group(C group),high sugar and high fat group(H group),control cells and H group cells were respectively stimulated with metformin(CM group and HM group).EDU staining was used to detect the cell proliferation;after osteogenesis and adi-pogenesis induction of BMSCs,alkaline phosphatase staining,alizarin red(O)staining and oil red O staining were respectively used to detect the osteogenic and adipogenic differentiation of the cells.The protein expression of Runx2 and OCN was detected by Western blot and immunofluorescence staining respectively.Results:Compared with the H group,in the HM group the cell proliferation ability was enhanced,the expression levels of Runx2 and OCN were increased(P＜0.05),the osteogenic differentiation ability was significantly in-creased,and the adipogenic differentiation ability was significantly decreased.Conclusion:In vitro,metformin promotes the prolifera-tion and osteogenic differentiation of rat jaw BMSCs cultured with a high sugar and high fat environment by increase of the expression of Runx2 and OCN.
关键词
二甲双胍/Runx2/OCN/成骨分化Key words
Metformin/Runx2/OCN/Osteogenic differentiation引用本文复制引用
基金项目
安徽高校自然科学研究项目(KJ2020A0617)
医院引进人才科研基金(YR202117)
出版年
2024
NETL
NSTL
万方数据