摘要
目的 探讨高迁移率族蛋白B1(HMGB1)及白介素(IL)-1β、IL-2R、IL-6、IL-8及肿瘤坏死因子α(TNF-α)与轻度胃肠炎伴良性惊厥(CwG)发病机制的关系.方法 选取2022年1月至2023年11月期间在江西省儿童医院就诊的CwG患儿30例(CwG组)和同期轻度急性胃肠炎(AGE)不伴有惊厥的患儿30例(对照组).采集2组急性期和恢复期的静脉血,采用酶联免疫吸附法(ELISA)检测血清HMGB1水平;采用化学发光法检测血清IL1β、IL2R、IL6、IL8、TNF-α水平.结果 在急性期,CwG组的血清HMGB1、IL-6、IL-8、TNF-α水平均显著高于对照组(P＜0.001),而血清IL-1β、IL-2R水平与对照组比较差异无统计学意义(P＞0.05).CwG组的惊厥发作次数、惊厥发作总时间、病程均与血清HMGB1、IL-6、IL-8、TNF-α水平成正相关(P＜0.05或P＜0.001),且血清HMGB1、IL-6、IL-8、TNF-α 水平之间相互呈正相关(P＜0.05 或 P＜0.001).CwG 组恢复期 HMGB1、IL-6、IL-8、TNF-α 水平均较急性期下降(P＜0.05);2组在恢复期时HMGB1、IL-1β、IL-2R、IL-6、IL-8、TNF-α水平比较差异无统计学意义(P＞0.05).结论 HMGB1介导的免疫炎症网络与CwG的发病过程密切相关,血清HMGB1、IL-6、IL-8、TNF-α水平与CwG急性期的惊厥严重程度及病程转归显著相关.
Abstract
Objective To investigate the inflammatory mechanism of benign convulsions with mild gastroenteritis(CwG)by detecting serum inflammatory factors in acute and convalescent CwG.Methods Thirty children diagnosed with CwG were selected as the case group,and 30 chil-dren with mild acute gastroenteritis without convulsion were selected as the control group.Venous blood was collected in the acute stage and convalescent stage of the two groups.High mobility group box 1(HMGB1)was detected by enzyme-linked immunosorbent assay(ELISA).The levels of serum IL1β,IL2R,IL6,IL8 and TNF-α were detected by chemiluminescence.Results The lev-els of HMGB1,IL-6,IL-8 and TNF-α in the acute stage of the case group were higher than those in the control group(P＜0.05),but there was no significant difference in serum IL-1β and IL-2R between the two groups(P＞0.05).The number of seizures,the total duration of seizures and course of disease were positively correlated with serum levels of HMGB1,IL-6,IL-8 and TNF-α in the acute stage of the case group(P＜0.05).The serum levels of HMGB1,IL-6,IL-8 and TNF-αwere positively correlated with each other(P＜0.05).The levels of HMGB1,IL-6,IL-8 and TNF-αin the recovery stage were decreased compared with those in the acute stage in the case group(P＜0.05).There was no significant difference in the levels of HMGB1,IL-1 β,IL-2R,IL-6,IL-8 and TNF-α between the case group and the control group during the recovery period(P＞0.05).Conclusion HMGB1-mediated immune inflammatory network is involved in the pathogenesis of CwG,and monitoring the changes of inflammatory factors such as HMGB1 is helpful for the clini-cal diagnosis and treatment of CwG.
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