Effects of curcumin on human hypertrophic scar fibroblasts and its mechanism based on endoplasmic reticulum stress PERK-ATF4-CHOP signaling pathway
Objective To investigate the effect of curcumin(Cur)on human hypertrophic scar fibroblasts(HSFbs)and its mecha-nism based on endoplastic stress of the PERK-ATF4-CHOP signal network.Methods From February 2022 to March 2023,patients who received plastic surgery treatment from the First Affiliated Hospital of Xinjiang Medical University were studied.Their hypertrophic scar tissue was obtained and human hypertrophic scar fibroblasts were cultured in vitro.The effect of different concertration Cur on the cell pro-liferation activity of HSFbs was measured by CCK-8 assay.According to the analysis of proliferative activity data,the experiment was di-vided into blank group,1/2 IC50 group,and IC50 group.Detection of cell proliferation activity using EDU method.Flow cytometry was used to detect the apoptosis ability of cells in each group after Cur intervention.The effect of Cur on cell cycle was detected by flow cytome-try after 24 hours of treatment.Scrscratch assto detect cell invasion and migration ability.Western blot Expression of the ER stress-related proteins PERK,ATF 4,CHOP,and Cleaved-Caspase-12 in the cells.Results As the concentration of Cur gradually increases,the cell survival rate of HSFbs gradually decreased and showed concentration dependence,with an IC50 value of 71.33 μmol/L.After the action of Cur on HSFbs24h,HSFbs was arrested in G1 phase.Cur intervention in the experimental group significantly inhibited the migration ability of HSFbs after 24 hours.The apoptosis of HSFbs was significantly promoted.Western blot experiment showed that Cur can significantly upregulate the protein expression level of PERK,ATF 4,CHOP and Cleaved-Caspase-12 in cells.Conclusion Cur can activate Endo-plasmic reticulum stress PERK-ATF4-CHOP signaling pathway,inhibit cell proliferation and migration of HSFbs,and promote cell apop-tosis of HSFbs.
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