Effect of body mass index on the cell composition of adipose tissue stromal vascular fraction
Objective To explore the impact of body mass index(BMI)on the cell composition of the stromal vascular fraction(SVF)obtained from human subcutaneous adipose.Methods In this study,abdominal subcutaneous adipose tissue was collected from 61 patients undergoing liposuction,following which SVF was isolated for flow cytometry.Based on BMI,patients were categorized into lean groups(BMI＜25 kg/m2)and overweight or obese groups(BMI ≥ 25 kg/m2).Results Flow cytometry results revealed significant re-ductions in relative percentages of ADSCs,adipose precursor cells,endothelial cells,M2 macrophages,and CD34+cell subsets of adipose tissue SVF in overweight and obese groups compared to the lean group(P＜0.05).However,there was no significant difference in relative percentages of endothelial progenitor cells,hematopoietic stem cells,monocytes,and pericytes between both groups(P＞0.05).Notably,the M1/M2 macrophage ratio significantly increased in the overweight and obese group(P＜0.05).Conclusion The cell composition of adi-pose tissue SVF varies significantly among different BMI groups.The proliferation,adipogenesis,angiogenesis,and regenerative functions of SVF in overweight and obese adipose tissues are impaired,which may further lead to adipose tissue dysfunction and affect the efficacy of SVF in clinical application.
Stromal vascular fraction(SVF)Adipose derived stem cells(ADSCs)Body mass index(BMI)Heterogeneity
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