缺氧微环境对转移性皮肤黑色素瘤预后及免疫治疗的影响
Effect of hypoxic microenvironment on prognosis and immunotherapy of metastatic cutaneous melanoma
杨文超 1秦瑞宁 1张俊瑕1
作者信息
- 1. 河西学院附属张掖人民医院 公卫科,甘肃 张掖 734000
- 折叠
摘要
目的 建立一种基于缺氧基因模型来评估皮肤黑色素瘤(skin cutaneous melanoma,SKCM)预后和免疫微环境.方法 自 2023 年 3-7 月,河西学院附属张掖人民医院公卫科通过检索公共数据库(TCGA、GEO、GTEx)查找SKCM病例,Cox回归分析筛选缺氧相关性状的基因.构建了一个包含 7 个缺氧基因的风险模型.GSEA用于比较两组的富集差异,使用CIBERSORT工具研究免疫细胞和基因特征之间潜在的相关性.结果 发现SKCM缺氧模型含有的 7 个基因(FBP1、GPI、BGN、SERPINE1、SDC4、PGM1、EGFR)高风险组患者的存活率低于低风险组.此外,9 种免疫细胞在高、低风险组患者的组织中有明显的浸润差异.结论 缺氧与SKCM患者的预后和免疫细胞浸润的发生率相关,这为个性化治疗的发展提供了免疫学视角.
Abstract
Objective To develop a hypoxia-based gene model to assess prognosis and immune microenvironment of skin cuta-neous melanoma(SKCM).Methods Public database(TCGA,GEO,GTEx)was used to search SKCM cases.Cox regression analysis was used to screen genes for hypoxia-related traits.A risk model containing seven hypoxia genes was constructed.GSEA was used to com-pare enrichment differences between the two groups,using the CIBERSORT tool to investigate potential correlations between immune cells and genetic signatures.Results Seven genes(FBP1,GPI,BGN,SERPINE1,SDC4,PGM1,EGFR)contained in the SKCM hypoxia model were found to have a lower survival rate in the high-risk group than in the low-risk group.In addition,we found significant differ-ences in the infiltration of nine immune cells in the tissues of patients in the high-and low-risk groups.Conclusion Hypoxia is associated with the prognosis and the incidence of immune cell infiltration in SKCM patients,which provides an immunological perspective for the de-velopment of personalized therapy.
关键词
皮肤黑色素瘤/缺氧/免疫治疗/公共数据库/生物信息学Key words
Melanoma of skin/Hypoxia/Immunotherapy/Public database/Bioinformatics引用本文复制引用
出版年
2024
NETL
NSTL
万方数据