Preliminary study on the related mechanism of Dnase1 interference of NETs on platelet-rich plasma accelerated wound healing in diabetic rats
Objective To investigate the impact of co-injection of deoxyribonuclease 1(Dnase1)and platelet-rich plasma(PRP)on wound healing in diabetic rats after degradation of neutrophil extracellular traps(NETs)by Dnase1 injection.Methods Four ulcer wound models were established in diabetic rats from May 2022 to July 2022,Huazhong University of Science and Technology Union Shen-zhen Hospital Animal Laboratory in collaboration with Topu Biological Experimental Center and randomly divided into 4 groups:Group A(Dnase+PRP):Dnase1 and PRP were sequentially injected subcutaneously at the edge of the wound.Group B(PRP):PRP was injected subcutaneously at the edge of the wound.Group C(Dnase):Dnase1 was injected subcutaneously at the edge of the wound.Group D(Con-trol):Normal saline was injected subcutaneously at the edge of the wound.Twelve rats from each group were selected on the 5th and 14th days to observe wound healing and granulation tissue growth.Wound tissues were collected,and histological examination using HE staining and immunohistochemistry was conducted to observe tissue growth.Tissue growth factors,inflammatory factors,and NETs-related indica-tors were detected and compared.Results After 5 days of treatment,there was no significant change in wound healing rate in each group.However,after 14 days of treatment,Group A showed the highest wound healing rate.The concentrations of vascular endothelial growth factor(VEGF)and transforming growth factor-β1(TGF-β1)in wound tissues of Group A and B were higher than those in the other two groups(P<0.05).The production of myeloperoxidase(MPO)and neutrophil elastase(NE)in wound tissues of Group A and C was lower than that in the other two groups(P<0.05).Conclusion Co-injection of Dnase1 and PRP can reduce the level of NETs in diabetic rat wounds,promote cell proliferation,angiogenesis,and thereby facilitate wound healing.
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