Assessment of an innovative preservation solution on rat hair follicle activity at low temperature
Objective To investigate the effect of insulin-like growth factor-1(IGF-1)and epidermal growth factor(EGF)inno-vative preservative on hair follicle activity of rat vibrissae under low temperature conditions.Methods From January 2021 to December 2023,the upper of hair follicle(UHF)from male SD rat vibrissae was used in the experiment.These UHF were equally divided into NS,IGF-1,EGF,and IGF-1+EGF preservation solutions groups.At 3,6 and 12 hours,the cultured UHF were analyzed by hematoxylin and eosin(HE)staining and alkaline phosphatase(ALP)activity.The UHF co-cultured with NS and IGF-1+EGF for 3 hours were further transplanted into the dorsum of nude mice.After 24 hours,the transplanted UHF was taken to observe by HE staining and ALP activity.Results In vitro preservation,HE staining results indicated that the cultured UHF at 3 hours,the follicle structure and cell arrangement were normal without obvious changes.At 12 hours,only the IGF-1+EGF group had intact follicle structure and clear organization.More-over,ALP values showed that the IGF-1+EGF group at 3,6 and 12 hours were significantly higher than the NS group(P＜0.001).Overall,ALP activity gradually decreased over time.In vivo transplantation,HE staining results showed that the IGF-1+EGF group were structural-ly distinct with orderly cell arrangements,compared to the NS group.ALP values were significantly higher than NS group(P＜0.05).Con-clusion The innovative preservation solutions containing IGF-1 and EGF under low temperatures,can effectively enhance the activity of UHF,and promote the success of hair follicle transplantation.
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