Phenotypic and cellular characteristics of adipose tissue prepared using mechanical shear force on human fat
Objective To analyze the effect of mechanical shear force on the final fat retention in the preparation of stromal vas-cular fraction(SVF)/extracellular matrix(ECM)gel from a cytological perspective.Methods Fat aspirates from patients undergoing lipo-suction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare the SVF/ECM gel.In the control group,fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts,while in the experimental group,Coleman fat was further separated by mechanical shear force to prepare SVF/ECM gel.The fat retention rate,cell composition and phenotype of the two groups were compared.The fat retention rate,cell composition and phenotype of the two groups were compared.Re-sults The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3,14,28,and 60 days following transplantation on the backs of nude mice.The viscosity of the fat was directly proportional to the shearing force.Although the mechanical isolation did not affect the total number of cells,it significantly decreased the number of living cells.Flow cytometry showed a greater num-ber of mesenchymal stem cells,supra-adventitial adipose stromal cells,and adipose-derived stem cells but lower number of endothelial progenitor cells in the SVF/ECM gel than in the Coleman fat.Conclusion Mechanical isolation of fat can increase the pluripotency of adipocytes,which can improve graft retention in cell therapy.
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