机械剪切力对人脂肪组织细胞表型及特性的影响
Phenotypic and cellular characteristics of adipose tissue prepared using mechanical shear force on human fat
叶媛 1邹敬江 1胡葵葵 1姜金豆 1郭炳阳 1李雄杰 1谭梅军1
作者信息
- 1. 广东省妇幼保健院医学美容科,广东 广州 511400
- 折叠
摘要
目的 分析脂肪胶制备过程中所受的剪切力对最终脂肪成活率的影响.方法 自2018年7月至2019年1月,广东省妇幼保健院医学美容科获取吸脂手术患者的脂肪,将其制备成标准的Coleman fat作为对照组,再将Coleman fat进一步利用机械剪切分离,制备成的基质血管片段(stromal vascular fraction,SVF)/细胞外基质(extracellular matrix,ECM)凝胶状(gel)脂肪(SVF/ECM gel)作为实验组.对这两种脂肪成活率、细胞组分及表型进行对比研究.结果 裸鼠背部移植后3、14、28、60d,SVF/ECM gel的成活率显著高于Coleman fat.脂肪的粘度与剪切力成正比.虽然剪切力不影响细胞总数,但它显著减少了活细胞的数量.流式细胞术显示,SVF/ECM gel中间充质干细胞、细胞外膜上脂肪基质细胞和脂肪来源干细胞(adipose-derived stem cells,ADSCs)数量较多,但内皮祖细胞数量较Coleman fat减少.结论 机械剪切力分离脂肪可以增加脂肪细胞的多能性,从而在细胞疗法中改善脂肪移植物成活率.
Abstract
Objective To analyze the effect of mechanical shear force on the final fat retention in the preparation of stromal vas-cular fraction(SVF)/extracellular matrix(ECM)gel from a cytological perspective.Methods Fat aspirates from patients undergoing lipo-suction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare the SVF/ECM gel.In the control group,fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts,while in the experimental group,Coleman fat was further separated by mechanical shear force to prepare SVF/ECM gel.The fat retention rate,cell composition and phenotype of the two groups were compared.The fat retention rate,cell composition and phenotype of the two groups were compared.Re-sults The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3,14,28,and 60 days following transplantation on the backs of nude mice.The viscosity of the fat was directly proportional to the shearing force.Although the mechanical isolation did not affect the total number of cells,it significantly decreased the number of living cells.Flow cytometry showed a greater num-ber of mesenchymal stem cells,supra-adventitial adipose stromal cells,and adipose-derived stem cells but lower number of endothelial progenitor cells in the SVF/ECM gel than in the Coleman fat.Conclusion Mechanical isolation of fat can increase the pluripotency of adipocytes,which can improve graft retention in cell therapy.
关键词
血管基质片段/细胞外基质的凝胶状脂肪/科尔曼脂肪/剪切力/组织再生/干细胞Key words
Vascular matrix fragment/SVF/ECM gel/Coleman fat/Shear force/Tissue regeneration/Stem cells引用本文复制引用
基金项目
广州市科技计划项目(202102020179)
出版年
2024
NETL
NSTL
万方数据