Effects of Propranolol on inhibition of HUVEC migration and invasion via the Notch signaling pathway
Objective To preliminarily elucidate the potential mechanisms of Propranolol in the treatment of infantile heman-gioma(IH)by investigating its effects on the migration and invasion of human umbilical vein endothelial cell(HUVEC)and the possible signaling pathways involved.Methods From August 2022 to January 2024,the HUVEC cell line was used as the research object to ob-serve the effects of Propranolol on cell migration and invasion ability before and after treatment by scratch test and Transwell test.Re-al-time fluorescent PCR and western blot assay were used to detect the effects of Propranolol on the Notch signaling pathway.The experi-mental group was divided into the Propranolol treatment group,Propranolol treatment group and Jagged1 treatment group,the untreated group was the blank control group.Results Propranolol significantly inhibited the invasion and migration of HUVEC in vitro.Following Propranolol treatment,the expression levels of Notch signaling components NICD1,Jagged1,and downstream effector Hes1 were markedly decreased,while the expression level of Notch1 was significantly upregulated.These changes were partially reversed by applying the Notch signaling activator Jagged1.Conclusion Propranolol significantly reduces the migration and invasion capabilities of HUVEC by inhibiting the Notch signaling pathway.These findings enhance our understanding of the molecular mechanisms underlying propranolol's therapeutic effects on IH.
PropranololHUVECNotch signaling pathwayMigration and invasionHemangioma
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