目的 利用在线数据库和生物信息学的方法,分析ZMYND8在皮肤黑色素瘤(malignant melanoma,MM)中的表达水平及其与MM发生发展、预后及免疫细胞浸润的相关性.方法 自2024年2-4月,联勤保障部队第九四○医院烧伤整形科采用GEPIA在线数据库(TCGA数据)分析多种正常组织与MM中ZMYND8表达情况,进一步利用GEO数据验证ZMYND8在MM中的表达差异;UALCAN数据库分析ZMYND8与MM患者预后的相关性;采用The Human Protein Atlas数据库分析ZMYND8在MM组织中的免疫组化表达;String数据库构建ZMYND8共表达基因的蛋白互作网络;采用TMER数据库分析ZMYND8表达与免疫浸润细胞相关性.结果 ZMYND8表达水平在MM组织中显著高于正常组织(P＜0.01);ZMYND8的高转录水平与性别、年龄、肥胖情况、淋巴转移和癌症分期阶段密切相关(P＜0.05);ZMYND8 mRNA表达水平与MM预后和免疫细胞浸润相关(P＜0.05).结论 ZMYND8在MM中高表达,与MM的发生发展、预后及免疫细胞浸润相关,可作为皮肤MM诊断、预后及药物治疗的潜在标志物.
Analysis of the correlation between ZMYND8 and the occurrence,prognosis and immune infiltration of malignant melanoma
Objective To use online databases and bioinformatics methods to analyze the expression level of ZMYND8 in malig-nant melanoma(MM)and its association with MM development,prognosis,and immune cell infiltration.Methods The GEPIA online database(TCGA data)was used to analyze the expression of ZMYND8 in a variety of normal tissues compared with the cancer tissues,and the GEO data was further used to confirm the distinctions in the expression of ZMYND8 in MM;the UALCAN database analyzed the corre-lation between ZMYND8 and the prognosis of MM patients;and The Human Protein Atlas database was used to to analyze the immunohis-tochemical expression of ZMYND8 in MM tissues;String database to establish the protein interactions network of ZMYND8 co-expressed genes;and TMER database to analyze the association between ZMYND8 expression and immune cells infiltrating.Results In MM tissues,the expression level of ZMYND8 was higher than that in normal tissues(P＜0.01);the high transcription level of ZMYND8 was highly cor-related with gender,age,obesity status,lymphatic metastasis,and cancer staging stage(P＜0.05);and the expression level of ZMYND8 mRNA was correlated with the prognosis of MM and immune cell infiltration(P＜0.05).Conclusion ZMYND8 is highly expressed in MM,and its correlation with MM development,prognosis,and immune cells infiltration may serve as a potential marker for the diagnosis,prognosis,and drug treatment of MM.
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