Protective effect of metformin on acute photodamage in nude mice
Objective To explore whether metformin has protective effect on acute photodamage of nude mice induced by ultra-violet.Methods The nude mice were randomly divided into blank group,MET group and control group.The blank group does not do any processing,the MET group and control group received 65 J/cm2 wave ultraviolet irradiation,at the same time the MET group was given in-traperitoneal injection of MET solution.Then,the skin appearance changes of nude mice were compared by gross observation,the skin pathological changes of nude mice were compared by HE staining and Masson staining,the ROS of skin tissue were analyzed and compared by flow cytometry,and the expressions of IL-1(3,IL-6,TNF-α and MMP-1 were detected by Western blot.Data analysis and mapping were performed using GraphPad Prism 8.3.Results After ultraviolet irradiation at 65 J/cm2 and injection,the back skin of nude mice in the MET group showed less lesions,such as dandruff,skin glossiness,local redness,ruptures,and crust-forming.H&E staining showed that compared with the blank group,the MET group and control group showed varying degrees of epidermal thickening,hyperkeratosis with parakeratosis of the cuticle,reduction of skin appendages,and infiltration and expansion of subcutaneous capillaries.The above perfor-mance was less severe in the MET group than that in the control group.The epidermal thickness in the MET group was significantly lower than that in the control group(P＜0.05).Masson staining showed that compared with the blank group,the blue-stained areas in the MET group and the control group were narrowed to varying degrees,the distribution was uneven,the blue-stained collagen was arranged disor-derly,and the continuity was interrupted.The above performance was also less severe in the MET group than in the control group.Flow cy-tometry showed that ROS in both MET and control groups was significantly higher than that in blank group(P＜0.05,P＜0.001),but the control group increased more(P＜0.01).Western blot showed that the relative expressions of IL-1(3(P＜0.01,P＜0.0001),IL-6(P＜0.05,P＜0.0001),TNF-α(P＜0.05.P＜0.0001)and MMP-1(P＜0.05,P＜0.0001)in the MET group and control group were higher than those in the blank group.Compared with control group,the expressions of IL-1(3(P＜0.05),IL-6(P＜0.05),TNF-α(P＜0.05)and MMP-1(P＜0.05)in MET group were lower.Conclusion Metformin has certain protective effect on acute photodamage in nude mice.
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